D. J. Krysan

Temporal dynamics of Candida albicans morphogenesis and gene expression reveals distinctions between in vitro and in vivo filamentation.

This paper compares the temporal dynamics of morphogenesis and gene expression during C. albicans filamentation in vitro and in vivo, identifying key differences in metabolic gene expression and hyphae-to-yeast transition rates.

The role of the C. albicans transcriptional repressor NRG1 during filamentation and disseminated candidiasis is strain-dependent

This study shows that the function of the C. albicans transcriptional repressor NRG1 varies by strain, affecting filamentation and virulence in different contexts.

Comparative dynamics of gene expression during in vitro and in vivo Candida albicans filamentation.

This study compares gene expression dynamics during in vitro and in vivo filamentation in Candida albicans, finding prolonged gene expression and adaptation in vivo.

The Candida albicans reference strain SC5314 contains a rare, dominant allele of the transcription factor Rob1 that modulates biofilm formation and oral commensalism

This paper identifies a rare, dominant SNP in the Rob1 transcription factor of C. albicans strain SC5314 that enhances biofilm formation and filamentation, contributing to its distinct phenotypic traits.

CWHM-974 is a fluphenazine derivative with improved antifungal activity against Candida albicans due to reduced susceptibility to multidrug transporter-mediated resistance mechanisms

Novel keto-alkyl-pyridinium antifungal molecules active in models of in vivo Candida albicans vascular catheter infection and ex vivo Candida auris skin colonization

This study identifies and characterizes novel keto-alkyl-pyridinium antifungal molecules with broad spectrum activity against fungi, showing efficacy in in vivo and ex vivo models of infection.

Candida albicans oropharyngeal infection is an exception to iron-based nutritional immunity

This study compares C. albicans gene expression in oral and kidney infections, finding it is iron-replete in oral tissue but iron-starved in the kidney, suggesting exemption from iron-based nutritional immunity in oral infections.

Intravital imaging-based genetic screen reveals the transcriptional network governing Candida albicans filamentation during mammalian infection

The paper uses intravital imaging to screen transcription factor mutants in C. albicans, identifying key regulators of filamentation during mammalian infection and revealing new insights into Efg1's function.

Candida albicans filamentation does not require the cAMP-PKA pathway in vivo

This study shows that the cAMP-PKA pathway is not required for C. albicans filamentation in vivo, despite being essential in vitro.

FKS1 is required for Cryptococcus neoformans fitness in vivo: application of copper-regulated gene expression to mouse models of cryptococcosis

This paper uses copper-regulated gene expression to study FKS1 in C. neoformans, showing reduced in vivo fungal burden with lower FKS1 expression.

Genetic interaction analysis reveals that Cryptococcus neoformans utilizes multiple acetyl-CoA-generating pathways during infection

This study identifies multiple acetyl-CoA-generating pathways in Cryptococcus neoformans essential for infection, showing synthetic lethality between ACL1 and ACS1 and reduced fitness in vivo.

Loss-of-function ROX1 mutations suppress the fluconazole susceptibility of upc2AΔ mutation in Candida glabrata, implicating additional positive regulators of ergosterol biosynthesis

The paper identifies ROX1 as a negative regulator of ergosterol biosynthesis genes in C. glabrata and shows that its loss-of-function suppresses fluconazole hypersusceptibility in upc2AΔ mutants.

In vivo complex haploinsufficiency-based genetic analysis identifies a transcription factor circuit regulating Candida albicans oropharyngeal infection and epithelial cell endocytosis

The paper identifies a transcription factor circuit regulating C. albicans oropharyngeal infection and epithelial cell endocytosis through genetic interaction analysis in a mouse model.

Intravital imaging of Candida albicans identifies differential in vitro and in vivo filamentation phenotypes for transcription factor deletion mutants

This paper uses intravital imaging to compare in vitro and in vivo filamentation phenotypes of C. albicans transcription factor mutants, finding distinct regulatory patterns in vivo.

Derivatives of the antimalarial drug mefloquine are broad spectrum antifungal molecules with activity against drug-resistant clinical isolates

This study identifies mefloquine derivatives as broad-spectrum antifungals effective against drug-resistant fungal isolates, with mechanisms involving mitochondrial and vacuolar disruption.

Host carbon dioxide concentration is an independent stress for Cryptococcus neoformans that affects virulence and antifungal susceptibility

This study identifies CO2 tolerance as a virulence trait in Cryptococcus neoformans, linking it to disease severity and antifungal drug susceptibility.