H. T. Soh

Theoretical framework and experimental validation of multiplexed analyte quantification using cross-reactive affinity reagents

This paper presents a mathematical framework and experimental validation to enable accurate multiplexed analyte quantification using cross-reactive affinity reagents.

Aptamer-antibody chimera sensors for sensitive, rapid and reversible molecular detection in complex samples

This paper introduces PANDAS, a sensor combining antibodies and aptamers for sensitive, rapid, and reversible molecular detection in complex samples, achieving 100-fold improved sensitivity for thrombin detection.

Real-Time Spatiotemporal Measurement of Extracellular Signaling Molecules Using an Aptamer Switch-Conjugated Hydrogel Matrix

The paper develops a hydrogel-based sensor to measure extracellular signaling molecules in real-time and demonstrates its use for cAMP in Dictyostelium discoideum.

A rapid ELISA platform with no sample preparation requirement

This paper introduces a rapid ELISA platform that requires no sample preparation, enabling quick quantification of protein biomarkers in blood samples.

An Antibody-Based Molecular Switch for Continuous Biosensing

This paper introduces an antibody-based molecular switch for continuous biosensing of biomarkers without sample preparation, demonstrating real-time detection of digoxigenin and cortisol with adjustable sensitivity.

Continuous optical detection of small-molecule analytes in complex biomatrices

This paper introduces an optical biosensor system for continuous detection of biomarkers in complex samples, achieving high sensitivity and long-term monitoring of dopamine and cortisol.

Extending the dynamic range of biomarker quantification through molecular equalization

This paper introduces a method to extend the dynamic range of biomarker quantification by equalizing molecular signals, achieving seven orders of magnitude in a single undiluted sample.

Binding and sequestration of poison frog alkaloids by a plasma globulin

The paper identifies a plasma globulin in poison frogs that binds and regulates alkaloid bioavailability, revealing a novel serpin-based mechanism for small molecule transport.

A high-throughput strategy for enhancing aptamer performance across different environmental conditions

The paper presents a high-throughput screening method to improve aptamer performance across different environmental conditions by testing mutants under target assay conditions.

Allosteric Regulation of Aptamer Affinity through Mechano-Chemical Coupling

This paper introduces a method to modulate aptamer affinity using a molecular clamp that destabilizes secondary structure through mechanical tension, demonstrating up to 65-fold affinity changes without prior structural knowledge.

Tailoring electrode surface charge to achieve discrimination and quantification of chemically similar small molecules with electrochemical aptamers

This paper demonstrates a method to distinguish and quantify chemically similar small molecules using electrochemical aptamers by tuning surface charge.

A modular intramolecular triplex photo-switching motif that enables rapid and reversible control of aptamer binding activity

This paper designs a DNA-based photoswitch that enables rapid and reversible control of aptamer binding activity through azobenzene isomerization.

Pre-equilibrium biosensors: A new approach towards rapid and continuous molecular measurements

This paper introduces a pre-equilibrium biosensor method to rapidly and sensitively measure ligand concentrations before equilibrium, demonstrating its application to continuous insulin monitoring.

Improved immunoassay sensitivity and specificity using single-molecule colocalization

The paper introduces SiMCA, a method using TIRF microscopy to improve immunoassay sensitivity and specificity by eliminating non-specific background through colocalization of fluorescent signals.

Directed Evolution of Aptamer Discovery Technologies

This paper describes four techniques to improve aptamer discovery, enabling efficient isolation of high-affinity and specific aptamers for challenging targets.

A system for multiplexed selection of aptamers with exquisite specificity without counter-selection

This paper introduces a high-throughput screening platform for generating highly specific aptamers without counter-selection, demonstrating its effectiveness with kynurenine metabolites.

Real-time monitoring of drug pharmacokinetics within tumor tissue in live animals

This paper develops an implantable sensor to monitor drug pharmacokinetics in tumor tissue in live animals, showing differences from systemic circulation that impact drug efficacy and safety.

A Metric for Quantifying the Resolution of Molecular Assays

The paper introduces a metric called resolution of molecular concentration (RMC) to quantify the discriminatory resolution of molecular assays, addressing limitations of existing metrics like limit of detection.

Accelerated electron transfer in nanostructured electrodes improves the sensitivity of electrochemical biosensors

This study identifies a mechanism by which nanostructured electrodes enhance electrochemical biosensor sensitivity through accelerated electron transfer, achieving up to 24-fold signal increase and four-fold lower limit-of-detection.

Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms

This paper develops indole-modified aptamers using FACS to specifically recognize protein glycoforms, achieving discrimination between structurally similar molecules differing only in glycan modifications.

Combinatorial Polyacrylamide Hydrogels for Preventing Biofouling on Implantable Biosensors

This study develops combinatorial polyacrylamide hydrogels that outperform current anti-biofouling coatings in preventing biofouling on biosensors.

Flow-cell based technology for massively parallelcharacterization of base-modified DNA aptamers

This paper introduces a flow-cell based system for high-throughput characterization of base-modified DNA aptamers using a modified Illumina MiSeq instrument.

Rational Design of Aptamer Switches with Programmable pH Response

This paper introduces a method to create pH-responsive aptamer switches by inserting orthogonal motifs, enabling tunable affinity changes across different pH conditions.

Measuring aptamer folding energy using a molecular clamp

This paper introduces a method to measure aptamer folding energy using a molecular clamp and validates it with experimental results for two aptamers.

Re-evaluating the conventional wisdom about binding assays

This paper challenges conventional wisdom about binding assays by re-evaluating principles of molecular recognition and proposes new design principles for optimal assay performance.

Continuous detection of glucose and insulin in live animals

This paper develops a biosensor capable of continuously measuring multiple analytes in live animals with high sensitivity and temporal resolution, demonstrating its use for glucose and insulin detection in diabetic rats.

A Dual-sensing DNA Nanostructure with an Ultra-broad Detection Range

This paper introduces a dual-mode DNA biosensor capable of detecting ATP over a five-order-of-magnitude range by combining fluorescence and absorbance readouts.

Independent Control of the Thermodynamic and Kinetic Properties of Aptamer Switches

This study introduces a design strategy to independently control the thermodynamic and kinetic properties of aptamer switches, enabling adjustable sensitivity and temporal resolution.

Click-PD: A Quantitative Method for Base-Modified Aptamer Discovery

This paper introduces click-PD, a method for discovering base-modified aptamers with high affinity and specificity using click chemistry and FACS screening.

High-Fidelity Nanopore Sequencing of Ultra-Short DNA Sequences

This paper introduces a method to accurately sequence ultra-short DNA sequences using nanopore sequencing, achieving SNV discrimination and variant quantification at low ratios.