A. K. Grosskopf

Kaposi's sarcoma-associated herpesvirus (KSHV) gB dictates a low-pH endocytotic entry pathway as revealed by a dual-fluorescent virus system and a rhesus monkey rhadinovirus expressing KSHV gB

This paper develops fluorescent KSHV strains and a hybrid RRV expressing KSHV gB to show low pH is required for KSHV membrane fusion during endocytosis.

Tim-1 and Tim-4 mediate entry of the human Kaposi's sarcoma-associated herpesvirus and the related rhesus monkey rhadinovirus

This paper identifies Tim-1 and Tim-4 as mediators of KSHV and RRV entry, showing their role in viral infection and highlighting their unique function compared to other herpesviruses.

Label-Free Composition Analysis of Supramolecular Polymer - Nanoparticle Hydrogels by Reversed-Phase Liquid Chromatography Coupled with a Charged Aerosol Detector

This paper develops a label-free RPLC-CAD method to analyze supramolecular hydrogel composition and drug payload, demonstrating its applicability for rheological and release kinetic studies.

Kaposi's sarcoma-associated herpesvirus glycoprotein K8.1 is critical for infection in a cell-specific manner and functions at the attachment step on keratinocytes

This study shows K8.1 is critical for KSHV infection in epithelial cells but not in B cells, functioning at the attachment step.

Injectable polymer-nanoparticle hydrogel for the sustained intravitreal delivery of bimatoprost

This paper develops an injectable polymer-nanoparticle hydrogel for sustained intravitreal delivery of bimatoprost, showing prolonged drug release and minimal inflammation in rabbits.

Stable High-Concentration Monoclonal Antibody Formulations Enabled by an Amphiphilic Copolymer Excipient

This paper introduces an amphiphilic copolymer excipient that enhances the stability of high-concentration monoclonal antibody formulations without affecting pharmacokinetics or injectability.

Yield Stress and Creep Control Depot Formation and Persistence of Injectable Hydrogels Following Subcutaneous Administration

This study investigates how hydrogel rheological properties affect depot formation and persistence after subcutaneous injection in mice.

Delivery of CAR-T Cells in a Transient Injectable Stimulatory Hydrogel Niche Improves Treatment of Solid Tumors

This paper develops an injectable hydrogel to co-deliver CAR-T cells and cytokines, enhancing solid tumor treatment in mice by creating a transient inflammatory niche.

Injectable Liposome Based Supramolecular Hydrogels for the Programmable Release of Multiple Protein Drugs

This paper develops injectable liposomal hydrogels that enable the programmed release of multiple protein drugs in vivo.

Interferon-Induced Transmembrane Proteins Inhibit Infection by the Kaposis Sarcoma-Associated Herpesvirus and the Related Rhesus Monkey Rhadinovirus in a Cell Type-Specific Manner.

This study identifies IFITM1 as a cell type-dependent inhibitor of KSHV and RRV infection, acting at the membrane fusion stage, and shows that HUVEC resist IFITM restriction despite high expression.

Injectable Nanoparticle-Based Hydrogels Enable the Safe and Effective Deployment of Immunostimulatory CD40 Agonist Antibodies

This paper develops injectable hydrogels to safely deliver CD40 agonist antibodies, reducing toxicity while maintaining anti-cancer efficacy in a melanoma model.

Consistent Tumorigenesis with Self-Assembled Hydrogels Enables High-powered Murine Cancer Studies

This paper introduces a hydrogel-based tumor inoculation method that reduces variability in murine tumor growth, enabling more efficient and reliable cancer studies.

Engineering Insulin Cold Chain Resilience to Improve Global Access

This paper develops amphiphilic copolymers to enhance insulin formulation resilience, enabling storage without refrigeration for over 6 months and improving global access to insulin.

SARS-CoV-2 and SARS-CoV spike-mediated cell-cell fusion differ in the requirements for receptor expression and proteolytic activation

This study compares the requirements for receptor expression and proteolytic activation in SARS-CoV-2 and SARS-CoV spike-mediated cell-cell fusion, revealing differences in their dependence on ACE2, TMPRSS2, and metalloproteases.

Hydrogel-based slow release of a receptor-binding domain subunit vaccine elicits neutralizing antibody responses against SARS-CoV-2

This study shows that slow release of an RBD subunit vaccine via hydrogel enhances neutralizing antibody responses against SARS-CoV-2 compared to bolus administration.

Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV)

This study identifies Plxdc1/2 as novel receptors for RRV, distinguishing it from KSHV and highlighting a conserved N-terminal motif in RRV gH/gL.

Combinatorial Polyacrylamide Hydrogels for Preventing Biofouling on Implantable Biosensors

This study develops combinatorial polyacrylamide hydrogels that outperform current anti-biofouling coatings in preventing biofouling on biosensors.

EphA7 functions as a receptor for cell-to-cell transmission of Kaposi’s sarcoma-associated herpesvirus into BJAB B cells and for cell-free virus infection by the related rhesus monkey rhadinovirus

This paper identifies EphA7 as the critical receptor for KSHV cell-to-cell transmission into BJAB B cells and for RRV cell-free infection, showing functional conservation between these viruses.