J. C. Boothroyd

Host MOSPD2 enrichment at the parasitophorous vacuole membrane varies between Toxoplasma strains and involves complex interactions

The paper investigates strain-specific variations in host MOSPD2 association with the Toxoplasma parasitophorous vacuole membrane and identifies factors influencing this interaction.

Cryogenic electron tomography reveals novel structures in the apical complex of Plasmodium falciparum

This study uses cryogenic electron tomography to reveal new structural details of the apical complex in Plasmodium falciparum merozoites, including interactions between rhoptries and other structures.

Coupling cryo-electron tomography with mixed-scale dense neural networks reveals re-organization of the invasion machinery of Toxoplasma gondii upon ionophore-stimulation

The paper uses cryo-electron tomography and mixed-scale dense neural networks to analyze the reorganization of Toxoplasma gondii's invasion machinery upon ionophore stimulation, revealing structural changes and evolutionary conservation with Plasmodium falciparum.

Emergent Actin Flows Explain Diverse Parasite Gliding Modes

This study uses single-molecule imaging and modeling to show that F-actin flows in parasites self-organize to produce diverse gliding motility modes.

Transcriptional signatures of clonally derived Toxoplasma tachyzoites reveal novel insights into the expression of a family of surface proteins.

The paper examines SRS gene expression in clonally derived Toxoplasma tachyzoites and finds that expression patterns are variable and not consistently inherited.

Proximity-labeling reveals novel host and parasite proteins at the Toxoplasma parasitophorous vacuole membrane

A single-parasite transcriptional atlas of Toxoplasma gondii reveals novel control of antigen expression

This study constructs a single-parasite transcriptional atlas of Toxoplasma gondii using scRNA-seq, revealing novel control mechanisms of antigen expression and developmental transitions.

Differential Impacts on Host Transcription by ROP and GRA Effectors from the Intracellular Parasite Toxoplasma gondii

This study analyzes the effects of ROP and GRA effector proteins from Toxoplasma gondii on host transcription using single-cell transcriptomics of uninfected-injected cells.

Co-immunoprecipitation with MYR1 identifies three additional proteins within the Toxoplasma parasitophorous vacuole required for translocation of dense granule effectors into host cells

The paper identifies three new proteins interacting with MYR1 in Toxoplasma that are essential for translocating effector proteins into host cells.

Translocation of dense granule effectors across the parasitophorous vacuole membrane in Toxoplasma-infected cells requires the activity of ROP17, a rhoptry protein kinase.

Toxoplasma gondii infection triggers chronic cachexia and sustained commensal dysbiosis in mice

This study shows that Toxoplasma gondii infection in mice causes chronic cachexia and sustained gut microbiota changes, but not all microbiota alterations are sufficient to cause cachexia.