T. C. Branon

Cilium proteomics reveals Numb as a positive regulator of the Hedgehog signaling pathway

This study identifies Numb as a ciliary protein that promotes Hedgehog signaling by facilitating Ptch1 exit from the cilium through endocytic mechanisms.

Shared and distinctive neighborhoods of emerin and LBR revealed by proximity labeling and quantitative proteomics

The paper uses proximity labeling and proteomics to identify proteins interacting with emerin and LBR at the inner nuclear membrane, revealing shared and distinct partners.

Proximity-labeling reveals novel host and parasite proteins at the Toxoplasma parasitophorous vacuole membrane

Proximity labeling at non-centrosomal microtubule-organizing centers reveals VAB-10B and WDR-62 as distinct microtubule regulators

The paper identifies VAB-10B and WDR-62 as distinct regulators of non-centrosomal microtubules using proximity labeling in C. elegans.

Split-TurboID enables contact-dependent proximity labeling in cells

Split-TurboID enables proximity labeling at organelle contact sites by splitting the TurboID enzyme and reconstituting it at these sites, identifying over 100 endogenous proteins.

LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is altered in Townes-Brocks Syndrome

The paper identifies LUZP1 as a regulator of primary cilia and actin cytoskeleton, showing its role in Townes-Brocks Syndrome through interactions with SALL1 and effects on ciliogenesis and Sonic Hedgehog signaling.

Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID

This study uses TurboID to identify protein interactions and cell type-specific organellar proteomes in Arabidopsis, revealing novel interactors of a transcription factor and subcellular proteomes.

Directed evolution of TurboID for efficient proximity labeling in living cells and organisms

The paper develops TurboID and miniTurbo, biotin ligase mutants with enhanced catalytic efficiency for proximity labeling in cells and organisms, significantly reducing labeling time and toxicity.