M. Trinidad

Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP

This study demonstrates that lipid nanoparticle-delivered thermostable Cas9 RNPs achieve high-efficiency genome editing in mouse liver and lung tissues.

Engineering self-deliverable ribonucleoproteins for genome editing in the brain

This study engineers self-deliverable CRISPR ribonucleoproteins for efficient genome editing in neural progenitor cells and mouse brain tissue.

Genome-wide Characterization of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi

The paper develops a CRISPRi-ART system to knock down protein expression in diverse bacteriophages and identifies essential genes, including many with unknown functions.

Genome editing in the mouse brain with minimally immunogenic Cas9 RNPs

This study demonstrates that minimally immunogenic Cas9 RNPs delivered via convection enhanced delivery can edit mouse striatal neurons with reduced immune responses compared to AAV-delivered Cas9.

Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells

This study investigates chromosome loss in CRISPR-Cas9-engineered T cells and shows a modified manufacturing process reduces it while preserving editing efficacy.

Precise Transcript Targeting by CRISPR-Csm Complexes

This study demonstrates that the CRISPR-Csm complex efficiently and precisely targets and degrades both nuclear and cytoplasmic transcripts in human cells with minimal off-target effects.

Targeted phosphoproteomics of the Ras signaling network reveal regulatory mechanisms mediated by oncogenic KRAS

This study uses targeted phosphoproteomics to analyze KRAS signaling network regulation in lung cancer cells, identifying unique phosphorylation changes and their roles in transcriptional silencing.