J. Wang

The paper introduces an efficient long fragment editing technique for scarless genome engineering in E. coli, enabling large-scale DNA insertion and deletion with high success rates.

This study identifies integrin β3 as a RACK1-interacting protein essential for PRRSV infection and NF-κB activation in Marc-145 cells, revealing a positive feedback loop involving NF-κB and ITGB3/RACK1.

This study shows PAT2 regulates autophagy in brown adipocytes by promoting vATPase assembly and lysosomal acidification in response to amino acid withdrawal.

This study investigates HMGB1's role in invasive C. albicans infection and shows that EP reduces HMGB1 levels, tissue damage, and mortality in infected mice.

This paper introduces a high-throughput drug screening method called CEBIT that detects biomolecular interactions and enzyme inhibitors by using phase-separated compartments.

This study identifies new epigenetic and genetic biomarkers for esophageal cancer using cfDNA and combines SALP-seq with machine learning.

This paper introduces GNEPS, a fluorescent sensor that detects intracellular GTP:GDP ratios in real-time, showing its utility in monitoring metabolic changes across cell types and organelles.

The paper uses ensemble docking with GaMD simulations and Autodock to identify potent allosteric modulators of the A1AR, showing improved performance over rigid docking methods.

This paper describes an automated peptide mapping protocol using a robotic liquid handling system to improve reproducibility and reduce hands-on time compared to manual methods.

This study shows that RIP1 kinase activity in macrophages drives steatohepatitis by promoting cell death and inflammation, and its inhibition reduces liver injury in NASH models.

This study examines microbiome changes in lung and gut of NSCLC patients and finds sputum microbiota better predicts disease and metastasis than gut microbiota, with machine learning models achieving 0.842 AUC.

This study improves subspecies identification of Mycobacterium abscessus complex using whole genome sequencing by developing a method based on rpoB, erm(41), and rpls genes, achieving 100% accuracy in public and clinical isolates.

The paper identifies mislabeled Drosophila stocks and shows how alignment-free phylogenomic analysis clarifies their true origins.

This study discovers that reversible covalent chemistry enhances PROTAC intracellular accumulation and target engagement, leading to improved efficacy.

This study investigates how urea and KI together denature protein structures through molecular dynamics simulations of TRPZIP4 in mixed solutions.

This study uses molecular dynamics simulations to show trehalose stabilizes protein structure by forming hydrogen bonds and replacing water molecules around the protein.

This study identifies host genetic variants associated with gut microbiome and examines causal relationships between gut microbiome and human complex diseases, revealing shared and distinct microbiome features.

The paper introduces the Kinase Chemogenomic Set (KCGS), a curated collection of 187 selective kinase inhibitors covering 215 human kinases, for use in cell-based screens.

This study develops a non-equilibrium model to analyze chromosome conformational changes during the cell cycle, revealing two-stage transitions and irreversible pathways.

The paper establishes a clinically and genomically annotated nerve sheath tumor biospecimen repository to support research on NF1-related tumors.

The paper introduces a microbial microdroplet culture system (MMC) that enables automated, high-throughput microbial cultivation and adaptive evolution, showing improved growth rates compared to conventional methods.

The paper introduces a genetically encoded 1D 1H-NMR probe for studying conformational changes in large membrane protein complexes, demonstrating its effectiveness with minimal protein and time.

This study describes a method to expand pancreatic islet clusters in vitro using hormones and chemicals, resulting in functional islet-like structures with cell type markers.

This study shows FLASH irradiation reduces intestinal injury while maintaining ovarian cancer treatment efficacy in preclinical models.

The paper analyzes the intestinal stem cell niche by combining bulk RNA sequencing and scRNA-seq to identify a network of cell types and signals involved in ISC regulation.

This study shows cells can sense long-range biomechanical forces through the matrix to initiate distant mechanical communication, enabling coordinated self-assembly.

This study shows that combining MEK and CDK4/6 inhibitors with immune checkpoint inhibitors improves pancreatic cancer treatment by enhancing T-cell infiltration and altering myeloid populations in preclinical models.

This study evaluates moxifloxacin efficacy against Mycobacterium abscessus in a zebrafish model, finding limited in vivo activity despite good in vitro results.

This study shows that copy number profiles are conserved in patient-derived xenografts (PDXs) through engraftment and passaging, indicating minimal impact from the mouse host on tumor evolution.

This study compares genome content and predatory phenotypes between Bdellovibrio sp. NC01 and B. bacteriovorus HD100, revealing differences in prey range and predation efficiency linked to horizontal gene transfer.