K. Schumacher

SPIRO - the automated Petri plate imaging platform designed by biologists, for biologists

SPIRO automates Petri plate imaging for plant phenotyping, improving precision and enabling dark condition assays with open-source accessibility.

Networked proteins redundantly interact with VAP27 and RABG3 to regulate membrane tethering at the vacuole and beyond

The paper shows NET4 and NET3 redundantly interact with RABG3 and VAP27 to regulate vacuole membrane tethering and growth in plants.

ATG8 delipidation is not universally critical for autophagy in plants

This study shows that ATG8 delipidation is essential for autophagy in Chlamydomonas but dispensable in Arabidopsis, highlighting evolutionary divergence in autophagy mechanisms.

Endometrial cyclin A2 deficiency is associated with human female infertility and is recapitulated in a conditional knockout mouse model

This study shows that reduced cyclin A2 expression in endometrial stromal cells is linked to infertility in women and is reproduced in a mouse model with conditional Ccna2 deletion.

Ribosome profiling reveals the fine-tuned response of Escherichia coli to mild and severe acid stress

This study uses ribosome profiling and RNA sequencing to analyze E. coli's response to mild and severe acid stress, identifying new genes, pathways, and sORFs.

Structure of V-ATPase from citrus fruit

This study determines the structure of plant V-ATPase from citrus fruit using SidK purification, revealing its ATP:H+ ratio and structural differences from yeast and mammalian enzymes.

Light at the end of the tunnel: FRAP assay reveals that plant vacuoles start as a tubular network

The paper reevaluates vacuolar biogenesis in Arabidopsis roots using 3D modeling and a customized FRAP assay, showing vacuoles form as a connected tubular network, not from MVBs.

Computational modeling and quantitative cell physiology reveal central parameters for the brassinosteroid-regulated early cell physiological processes linked to elongation growth of the Arabidopsis root

The paper uses computational modeling and quantitative cell physiology to identify key parameters and components involved in brassinosteroid-regulated cell elongation in Arabidopsis root epidermis.

CLCd and CLCf act redundantly at the TGN/EE and prevent acidification of the Golgi stack

This study shows CLCd and CLCf prevent TGN/EE hyper-acidification by regulating V-ATPase distribution, essential for male gametophyte development.

Pharmacological and genetic manipulations of Ca2+ signaling have contrasting effects on auxin-regulated trafficking

The paper investigates how pharmacological and genetic manipulations of Ca2+ signaling affect auxin-regulated PIN trafficking, finding contrasting results between exogenous Ca2+ manipulations and genetic mutants.

The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant specific motif and acts in a partially redundant manner with the tonoplast enzyme

This study identifies a seed plant-specific motif in Arabidopsis VHA-a1 that targets the V-ATPase to the TGN/EE and shows partial functional redundancy with tonoplast-localized VHA-a2 and VHA-a3.

Subtilase-mediated activation of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway

The paper shows that CLEL6 and CLEL9 peptides in Arabidopsis undergo multiple processing steps in the secretory pathway involving subtilases SBT6.1 and SBT3.8.

Dual-sensing genetically encoded fluorescent indicators resolve the spatiotemporal coordination of cytosolic abscisic acid and second messenger dynamics in Arabidopsis

The paper develops dual-sensing fluorescent indicators to analyze ABA and second messenger dynamics in Arabidopsis roots, revealing their spatiotemporal coordination during stress responses.

Pathogen-induced pH changes regulate the growth-defense balance of plants

This study shows that pathogen-induced pH changes regulate plant growth and defense by modulating cellulose synthesis and fungal pathogenicity through COMPANION OF CELLULOSE SYNTHASE proteins.

Pyrophosphate modulates stress responses via SUMOylation

This study shows that pyrophosphate modulates stress responses by inhibiting SUMOylation, affecting cold and heat stress sensitivity in plants and yeast.

NHX-type Na+(K+)/H+ antiporter activity is required for endomembrane trafficking and ion homeostasis in Arabidopsis thaliana

This study shows that NHX5 and NHX6 antiporters are essential for endosomal ion and pH homeostasis, affecting trafficking and motility but not membrane protein secretion in Arabidopsis.