M. G. Gutierrez

Physiologic media renders human iPSC-derived macrophages permissive for M. tuberculosis by rewiring organelle function and metabolism

This study shows that using a human plasma-like medium enhances M. tuberculosis replication in iPSC-derived macrophages by altering lipid metabolism and organelle function.

LRRK2 kinase dependent and independent function on endolysosomal repair promotes macrophage cell death

This study identifies LRRK2-G2019S-dependent lysosomal membrane repair defects in macrophages leading to increased cell death.

Lysosomal damage drives mitochondrial proteome remodelling and reprograms macrophage immunometabolism

This study shows lysosomal damage leads to mitochondrial proteome changes and metabolic reprogramming in macrophages, affecting immune responses to infections.

Visualizing pyrazinamide action by live single cell imaging of phagosome acidification and Mycobacterium tuberculosis pH homeostasis

This paper uses live-cell imaging to show that pyrazinamide disrupts Mycobacterium tuberculosis intracellular pH homeostasis, unlike other antibiotics, and that this effect depends on host endolysosomal acidification and PncA activity.

Intracellular localisation of Mycobacterium tuberculosis affects antibiotic efficacy

This study investigates how the intracellular localization of Mycobacterium tuberculosis affects the efficacy of the antibiotic pyrazinamide (PZA) using multimodal microscopy techniques.

LRRK2-dependent Rab GTPase phosphorylation in response to endolysosomal damage depends on macrophage differentiation

This study shows that LRRK2-dependent Rab GTPase phosphorylation in response to endolysosomal damage varies between macrophage subsets differentiated with M-CSF or GM-CSF.

The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infection

The paper shows that bedaquiline reprograms macrophages to enhance bactericidal activity against bacteria through metabolic and lysosomal pathways.

Elevated LRRK2 and α-synuclein levels in CSF of infectious meningitis patients

The paper finds elevated levels of LRRK2 and α-synuclein in CSF of infectious meningitis patients, suggesting a link between neuroinflammation and infection.

Mycobacterium tuberculosis cording in the cytosol of live lymphatic endothelial cells

This study shows that Mycobacterium tuberculosis forms intracellular cords in human lymphatic endothelial cells, evading xenophagy via the ESX-1 system and PDIM lipid.