P. B. Williams

Improving Nitrofurantoin Resistance Prediction in Escherichia coli from Whole Genome Sequence by Integrating NfsA/B Enzyme Assays

The paper identifies specific amino acid substitutions in NfsA and NfsB that reduce enzyme activity and cause nitrofurantoin resistance in E. coli, but finds that WGS alone cannot reliably predict resistance due to functional silent mutations and downregulated gene expression.

Harvesting and amplifying gene cassettes confers cross-resistance to critically important antibiotics

The paper shows that gene cassettes in an integron, amplified by IS26, confer resistance to amikacin and piperacillin/tazobactam, often leaving meropenem as the last-resort antibiotic.

Identification and characterisation of Klebsiella pneumoniae and Pseudomonas aeruginosa clinical isolates with atypical β-lactam susceptibility profiles using Orbitrap liquid chromatography-tandem mass spectrometry

The paper uses proteomics and whole genome sequencing to characterize clinical isolates of Klebsiella pneumoniae and Pseudomonas aeruginosa with atypical β-lactam susceptibility profiles, expanding predictive rules for antibiotic resistance.

Limited Phylogenetic Overlap Between Fluoroquinolone-Resistant Escherichia coli Isolated on Dairy Farms and those Causing Bacteriuria in Humans Living in the Same Geographical Region

The paper investigates the link between fluoroquinolone-resistant E. coli on dairy farms and human bacteriuria in the same region, finding limited phylogenetic overlap and identifying farm use patterns as risk factors.

Trade-Offs Between Antibacterial Resistance and Fitness Cost in the Production of Metallo-β-Lactamase by Enteric Bacteria Manifest as Sporadic Emergence of Carbapenem Resistance in a Clinical Setting.

The paper shows how meropenem resistance via NDM-1 in Enterobacteriales involves trade-offs between resistance and fitness, leading to sporadic resistance emergence in clinical settings.