K. J. Heesom

Improving Nitrofurantoin Resistance Prediction in Escherichia coli from Whole Genome Sequence by Integrating NfsA/B Enzyme Assays

The paper identifies specific amino acid substitutions in NfsA and NfsB that reduce enzyme activity and cause nitrofurantoin resistance in E. coli, but finds that WGS alone cannot reliably predict resistance due to functional silent mutations and downregulated gene expression.

Harvesting and amplifying gene cassettes confers cross-resistance to critically important antibiotics

The paper shows that gene cassettes in an integron, amplified by IS26, confer resistance to amikacin and piperacillin/tazobactam, often leaving meropenem as the last-resort antibiotic.

Roles for CEP170 in cilia function and dynein-2 assembly.

This study identifies CEP170 as a protein interacting with dynein-2, showing its role in dynein-2 assembly and cilia function through effects on intraflagellar transport and Hedgehog signaling.

Structural basis for coupling of the WASH subunit FAM21 with the endosomal SNX27-Retromer complex

This study defines how FAM21 couples SNX27-Retromer to the WASH complex through motif-based interactions essential for endosomal recycling.

Recombinant SARS-CoV-2 lacking initiating and internal methionine codons within ORF10 is attenuated in vivo

This study shows that a recombinant SARS-CoV-2 virus lacking ORF10 start and internal methionine codons is attenuated in hamsters, with reduced lower respiratory tract viral titre and increased reversion to wild-type.

Alterations in platelet proteome signature and impaired platelet integrin αIIbβ3 activation in patients with COVID-19

This study identifies altered platelet proteome and impaired integrin αIIbβ3 activation in COVID-19 patients, linked to increased platelet-neutrophil aggregates and thromboinflammation.

Small Molecule G-quadruplex Ligands are Antibacterial Candidates for Gram-negative Bacteria

The paper identifies a G-quadruplex ligand, L9, as a potential antibacterial agent against multi-drug resistant E. coli, showing superior activity and distinct mechanism compared to existing ligands.

Characterizing the polarization continuum of macrophage subtypes M1, M2a and M2c

This study characterizes four macrophage subtypes using proteomics and functional assays, showing M2c macrophages upregulate pro-inflammatory markers and dexamethasone induces partial repolarization from M1 to M2c.

Chemical degradation of BTK/TEC as a novel approach to inhibit platelet function and thrombosis.

This study shows that chemical degradation of BTK/TEC in human platelets reduces thrombotic function without affecting PAR-1-mediated responses.

Identification and characterisation of Klebsiella pneumoniae and Pseudomonas aeruginosa clinical isolates with atypical β-lactam susceptibility profiles using Orbitrap liquid chromatography-tandem mass spectrometry

The paper uses proteomics and whole genome sequencing to characterize clinical isolates of Klebsiella pneumoniae and Pseudomonas aeruginosa with atypical β-lactam susceptibility profiles, expanding predictive rules for antibiotic resistance.

ESCPE-1 Mediates Retrograde Endosomal Sorting of the SARS-CoV-2 Host Factor Neuropilin-1

The paper identifies Neuropilin-1 as a retrograde cargo of ESCPE-1 and shows ESCPE-1 mediates its trafficking, potentially facilitating SARS-CoV-2 infection.

Crosstalk between β-catenin and WT1 signalling activity in acute myeloid leukemia

This study identifies a novel interaction between β-catenin and WT1 in AML, showing they influence each other's expression and signaling activity, which may inform therapeutic strategies.

OmpF Downregulation Mediated by Sigma E or OmpR Activation Confers Cefalexin Resistance in Escherichia coli in the Absence of Acquired β-Lactamases.

This study identifies OmpF downregulation via Sigma E or OmpR activation as a mechanism for cefalexin resistance in E. coli without β-lactamase production.

Green Fluorescent Carbon Dots as Targeting Probes for LED-Dependent Bacterial Killing

This paper demonstrates that green fluorescent carbon dots can label and kill Gram-positive and Gram-negative bacteria when combined with LED light exposure.

Resistance to aztreonam in combination with non-β-lactam β-lactamase inhibitors due to the layering of mechanisms in Escherichia coli identified following mixed culture selection.

The paper identifies layered resistance mechanisms in Escherichia coli to aztreonam combined with β-lactamase inhibitors through mixed culture selection.

Trade-Offs Between Antibacterial Resistance and Fitness Cost in the Production of Metallo-β-Lactamase by Enteric Bacteria Manifest as Sporadic Emergence of Carbapenem Resistance in a Clinical Setting.

The paper shows how meropenem resistance via NDM-1 in Enterobacteriales involves trade-offs between resistance and fitness, leading to sporadic resistance emergence in clinical settings.

The type 2 diabetes gene product STARD10 is a phosphoinositide binding protein that controls insulin secretory granule biogenesis

This study identifies STARD10 as a phosphoinositide binding protein that regulates insulin granule biogenesis in beta-cells by affecting phosphatidylinositol levels and proinsulin processing.

A combined proteomics and Mendelian randomization approach to investigate the effects of aspirin-targeted proteins on colorectal cancer

This study combines proteomics and Mendelian randomization to identify aspirin-targeted proteins associated with reduced colorectal cancer risk.

Variability in carbapenemase activity of intrinsic OxaAb (OXA-51-like) beta-lactamase enzymes in Acinetobacter baumannii

This study measures carbapenem susceptibility variability in OxaAb variants of Acinetobacter baumannii using WGS and LC-MS/MS, finding that ISAba1 promoter-driven overexpression of specific substitutions confers resistance.

Proteomic Investigation of the Signal Transduction Pathways Controlling Colistin Resistance in Klebsiella pneumoniae.

This study uses proteomics to investigate how mutations in MgrB, PhoPQ, and PmrAB affect Arn protein production and colistin resistance in Klebsiella pneumoniae.

Characterisation of AmpC Hyper-Producing Escherichia coli from Humans and Dairy Farms Collected in Parallel in the Same Geographical Region

This study characterizes AmpC hyper-producing E. coli from dairy farms and humans, identifies risk factors, and finds no evidence of zoonotic transmission.

TonB dependent uptake of β-lactam antibiotics in the opportunistic human pathogen Stenotrophomonas maltophilia.

The paper shows that mutations in TonB confer ceftazidime resistance in S. maltophilia, challenging the belief that β-lactams only enter Gram-negative bacteria via passive diffusion.

Prediction of fluoroquinolone susceptibility directly from whole genome sequence data using liquid chromatography-tandem mass spectrometry to identify mutant genotypes.

The paper predicts fluoroquinolone susceptibility in K. pneumoniae using whole genome sequence data and protein abundance measurements from liquid chromatography-tandem mass spectrometry.

The Envelope Proteome Changes Driven By RamA Overproduction in Klebsiella pneumoniae That Enhance Acquired β-Lactam Resistance

This study shows RamA overproduction in K. pneumoniae enhances β-lactam resistance by increasing efflux pumps and reducing OmpK35 porin, without altering β-lactamase levels.