P. Nissen

Conserved N-terminal Regulation of the ACA8 Calcium Pump with Two Calmodulin Binding Sites

This study investigates the N-terminal regulation of ACA8 calcium pump in A. thaliana, showing that residues 20-35 and Phe32 are critical for autoinhibition and calmodulin activation.

Activation and substrate specificity of the human P4-ATPase ATP8B1

This study presents cryo-EM structures and functional analysis of the human ATP8B1-CDC50A complex, revealing its mechanism of action, substrate specificity, and regulation by phosphoinositides.

Variations of a theme - crystal forms of the amino acid transporter MhsT

The paper reports on the crystallization of MhsT with different substrates, revealing varied crystal packing arrangements and challenges in structure determination due to crystal imperfections.

Monomeric α-Synuclein activates the Plasma Membrane Calcium Pump

This study shows that monomeric α-synuclein activates the plasma membrane calcium pump (PMCA) in neurons, enhancing calcium expulsion through interactions with acidic phospholipids and membrane anchoring.

Activation and inhibition of the C-terminal kinase domain of p90 ribosomal S6 kinases

The paper characterizes the activation mechanism of the C-terminal kinase domain of RSKs and develops constitutively active variants for inhibitor testing.

Cryo-EM structure of the human NKCC1 transporter reveals mechanisms of ion coupling and specificity

The paper presents a cryo-EM structure of human NKCC1, revealing ion binding sites and mechanisms of ion coupling and specificity.

The beryllium fluoride form of the Na+,K+-ATPase and the intermediates of the functional cycle

The paper presents a crystal structure of the Na+,K+-ATPase in the E2-BeFx form, revealing structural insights into its functional cycle and ion binding mechanisms.

Autoinhibition and regulation by phosphoinositides of ATP8B1, a human lipid flippase associated with intrahepatic cholestatic disorders

This study investigates the autoinhibition and regulation of ATP8B1, a lipid flippase, through functional studies and cryo-EM structure analysis, revealing the role of phosphoinositides and terminal segments in its activity.

The main activatory and tension-sensitive transitions occur within Prozac sensitive down-states of the potassium selective TREK-2 channel

This study identifies two tension-sensitive transitions in TREK-2 channels, showing that the main activation occurs within down conformations, while the down-to-up transition provides additional activation.

Histidine-rich Ca2+-binding protein stimulates the transport cycle of SERCA through a conformation-dependent fuzzy complex.

This study shows HRC stimulates SERCA by binding in a conformation-dependent fuzzy complex, stabilizing the end of the SERCA transport cycle.

Computational Studies of Substrate Transport and Specificity in a Phospholipid Flippase

The paper uses molecular dynamics simulations to study phospholipid transport and specificity in a flippase, revealing a preference for phosphatidylserine and insights into substrate selectivity mechanisms.

The crystal structure of the Ca2+-ATPase 1 from Listeria monocytogenes reveals a pump primed for dephosphorylation

The paper presents crystal structures of LMCA1, revealing its primed state for dephosphorylation and a single Ca2+-binding site, with insights into its transport mechanism.

Role of transmembrane helix 6 in substrate recognition of the amino acid transporter MhsT

The paper investigates how the flexibility of a GMG motif in TM6 of MhsT enables substrate recognition of different amino acid sizes by shaping the binding site.

Namdinator - Automatic Molecular Dynamics flexible fitting of structural models into cryo-EM and crystallography experimental maps

Namdinator automates Molecular Dynamics Flexible Fitting to improve structural models in cryo-EM and crystallography maps, achieving model improvements in 87% of tested cases.

Structures of the heart specific SERCA2a Ca2+-ATPase

The paper presents the first crystal structures of SERCA2a, revealing structural and regulatory differences from SERCA1a and providing insights into its function in cardiac muscle.