S. Bakshi

Single-cell imaging of the lytic phage life cycle in bacteria

This study uses time-lapse imaging to analyze phage infection dynamics in bacteria, revealing heterogeneity and its impact on phage fitness.

Protein Translation Can Fluidize Bacterial Cytoplasm

This study shows that protein synthesis, particularly ribosome dynamics, enhances fluidity in bacterial cytoplasm by counteracting its glassy nature through metabolic activity.

Quantitative microbiology with widefield microscopy: navigating optical artefacts for accurate interpretations

This study identifies artefacts in 2D widefield microscopy of microbial cells caused by diffraction and projection effects, and proposes experimental designs and machine learning methods to improve quantification accuracy.

Timing and magnitude of the type-I interferon response are determinants of disease tolerance in arbovirus infection

This study shows that the timing and magnitude of the type-I interferon response determine disease tolerance in bluetongue virus infection between sheep and cattle.

Zoonotic avian influenza viruses evade human BTN3A3 restriction

This study identifies BTN3A3 as a human restriction factor that inhibits avian influenza viruses but not human IAVs, with resistance linked to specific NP mutations.

SyMBac: Synthetic Micrographs for Accurate Segmentation of Bacterial Cells using Deep Neural Networks

SyMBac generates synthetic bacterial micrographs for deep learning segmentation, outperforming human-annotated data in accuracy and robustness across imaging conditions.

Capsule protects against intracellular killing and enables vascular endothelial cell translocation during invasive pneumococcal disease

This study shows that pneumococcal capsule protects bacteria from oxidative stress in host cells and enhances disease severity by promoting intracellular survival and translocation.

Transcriptomic analysis of naïve human embryonic stem cells cultured in three-dimensional PEG scaffolds

This study shows that 3-D PEG scaffolds maintain the naive state of human ESCs and alter gene expression compared to 2-D culture.

Tracking bacterial lineages in complex and dynamic environments with applications to growth control and persistence

The paper develops a platform to track bacterial lineages during growth and shows changes in growth modes and cell size distribution across exponential, stationary, and post-stationary phases in Escherichia coli and Bacillus subtilis.