S. Rouskin

Diverse Database and Machine Learning Model to narrow the generalization gap in RNA structure prediction

The paper introduces a diverse RNA structure database and a deep learning model, eFold, which improves RNA secondary structure prediction by emphasizing structural diversity over database size.

Telomerase RNA structural heterogeneity in living human cells detected by DMS-MaPseq

This study identifies a misfolded CR4/5 conformation in hTR that is not required for hTERT binding and is detrimental to telomerase activity.

Structure of pre-miR-31 reveals an active role in Dicer processing

The paper determines the structure of pre-miR-31 and identifies structural elements that influence Dicer processing.

3D RNA-scaffolded wireframe origami

This paper designs, fabricates, and characterizes 3D RNA-scaffolded wireframe origami structures using different RNA sequences, revealing structural insights and demonstrating the potential for functional RNA scaffolds.

Recurrent emergence of carbapenem resistance in Klebsiella pneumoniae mediated by an inhibitory ompK36 mRNA secondary structure

This study identifies a synonymous mutation in ompK36 that increases carbapenem resistance in Klebsiella pneumoniae by causing translational suppression.

An intranasal ASO therapeutic targeting SARS-CoV-2

This paper develops an intranasal ASO therapy targeting SARS-CoV-2 RNA that effectively prevents viral replication in multiple models and variants.

Translational activation by an alternative sigma factor in Bacillus subtilis

The paper shows that the sigma factor σB in Bacillus subtilis induces translation of its regulon genes by relieving RNA secondary structures, leading to significant translation efficiency differences compared to σA.

Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cells

This study identifies RNA secondary structures of the full SARS-CoV-2 genome in infected cells, revealing alternative conformations that influence frameshifting rates.