M. Bathe

Evaluation of non-modified wireframe DNA origami for acute toxicity and biodistribution in mice

This study evaluates the acute toxicity and biodistribution of non-modified wireframe DNA origami nanoparticles in mice, finding no toxicity and minimal immunotoxicity.

Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds

This study shows DNA origami scaffolds enhance antibody responses to SARS-CoV-2 RBD without inducing immune memory against the scaffold itself, compared to protein-based VLPs.

3D RNA-scaffolded wireframe origami

This paper designs, fabricates, and characterizes 3D RNA-scaffolded wireframe origami structures using different RNA sequences, revealing structural insights and demonstrating the potential for functional RNA scaffolds.

Innate Immune Stimulation using 3D Wireframe DNA Origami

This study investigates how 3D wireframe DNA origami stimulate the TLR9 pathway, finding that multivalent CpG display enhances immune activation while bare structures do not.

Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cells

This study identifies RNA secondary structures of the full SARS-CoV-2 genome in infected cells, revealing alternative conformations that influence frameshifting rates.

Controlling wireframe DNA origami nuclease degradation with minor groove binders

This study demonstrates that minor groove binders can protect wireframe DNA origami from endonuclease degradation, enhancing their stability in biological environments.

Role of nanoscale antigen organization on B-cell activation probed using DNA origami

This study uses DNA origami to investigate how antigen organization affects B-cell activation, finding that optimal spacing and valency enhance signaling.

Rapid Prototyping of Wireframe Scaffolded DNA Origami using ATHENA

ATHENA is an open-source software that automates wireframe DNA origami design using 2D/3D geometries and 2HB/6HB edges, enabling sequence generation and integration with simulation tools.

Random access DNA memory in a scalable, archival file storage system

This paper demonstrates a method to enable random access of DNA-encoded files in large-scale archival storage by using silica capsules with barcode labels for efficient file selection.

Bioproduction of single-stranded DNA from isogenic miniphage

This paper demonstrates the scalable production of custom-length single-stranded DNA (cssDNA) using engineered miniphage in E. coli, enabling applications in DNA origami and archival data storage.

Enzymatic synthesis of gene-length single-stranded DNA

This paper develops an enzymatic method to synthesize long ssDNA sequences (>15 kb) with controlled nucleotide composition for therapeutic and imaging uses.