T. Wu

The paper computationally designs and experimentally validates potent inhibitors of the SARS-CoV-2 Nsp3 Macrodomain, identifying 160 ligands and 152 crystal structures with submicromolar affinity.

The paper introduces a raMVR microscope that achieves 6D super-resolution imaging of single molecules with high precision in 3D position and orientation.

This study shows HDAC3 is essential for pDC development and hematopoietic progenitor homeostasis, with HDAC3 repressing cDC1-associated genes to promote pDC differentiation.

This study shows that elevated membrane tension induces the formation of giant clathrin-coated pits (GCPs) in fruit fly embryos, which differ in size and internalization dynamics from canonical clathrin-coated pits.

This study quantifies neoantigen-mediated immunoediting stages in cancer evolution across tumor types, showing elimination and escape signals predict immunotherapy response.

The paper quantifies genetic correlations among mutualism traits in Turnera ulmifolia to assess their evolutionary implications.

This study shows that extrachromosomal DNA (ecDNA) is linked to reduced immune cell infiltration and antigen presentation, suggesting it helps cancer cells evade immune surveillance.

This paper develops bright fluorophores and uses heavy water to improve fluorescence imaging of deep-buried hollow organs, achieving better visualization and diagnosis.

This paper introduces self-confocal NIR-II fluorescence microscopy systems for in vivo imaging, improving adjustability and enabling cerebrovascular and hepatic imaging.

The paper introduces TRAPS-seq, a method to measure single-cell protein secretion, phenotypes, and transcriptomes, revealing limited correlation between cytokine secretion and transcript abundance in T cells.

This study develops an intranasal vaccine against SARS-CoV-2 using an influenza virus vector and shows it provides rapid, prolonged, and broad protection against multiple variants in hamsters.

The paper presents a method to dual site-specifically label proteins using tryptophan auxotrophic E. coli and 5-hydroxytryptophan, demonstrated with E. coli release factor 1.

This paper engineers BmoR mutants to create alcohol biosensors with high specificity and sensitivity for detecting specific alcohols in the presence of background ethanol.

This paper introduces a radially and azimuthally polarized microscope to measure single-molecule orientation and dynamics with high precision, revealing cholesterol-induced changes in membrane behavior.

The paper shows antigen-derived peptides activate IRE1α in dendritic cells, reducing MHC-I expression and cross-presentation, and demonstrates that IRE1 inhibition enhances anti-tumor immunity.

This study constructs high-resolution spatial transcriptomes of primary liver cancers to analyze spatial architecture and identify tumor microenvironment features.

The paper defines and extends the near-infrared biological window for improved in vivo fluorescence imaging, proposing new regions and demonstrating enhanced imaging capabilities.

This study uses computational fluid dynamics to model cough particle transport in an aircraft cabin, finding that 80% of particles are removed within 2.6 minutes and highest exposure is 0.3% of expelled mass.

The paper presents the cryo-EM structure of the human Nav1.5-E1784K variant and identifies a mutational hotspot in fast inactivation segments linked to LQT3.

The paper identifies new chemical fragments binding to the SARS-CoV-2 Nsp3 macrodomain through crystallographic screening and computational docking, validating their binding with biophysical techniques.

This study introduces TRuST, a label-free method for T cell reconstitution using side scatter, showing improved enrichment and expansion capacity of specific T cell subsets.

This paper introduces a method to measure bacterial surface charge density using second harmonic light scattering and finds that Gram-negative bacteria have seven times higher negative charge density than Gram-positive bacteria.

This paper develops a bioelectric neural interface using implantable electrodes and AI to enable intuitive control of a high-DOF prosthetic hand for amputees.

This study identifies a novel oxidase in Alcaligenes sp. HO-1 that converts hydroxylamine to N2, revealing a previously unknown enzymatic pathway for nitrogen cycling.

This study integrates proteomic data from human and mouse macrophages to analyze posttranslational modifications in the inflammatory response, identifying PTM crosstalk and novel inflammatory proteins.

The paper identifies biochemically neutral positions in human liver pyruvate kinase by analyzing evolutionary patterns and functional outcomes of amino acid substitutions.

The paper quantifies historical and future habitat loss for Asian elephants across multiple scenarios, showing significant declines and the need for equitable policies.

This study creates a single-cell transcriptome atlas of a non-human primate to explore SARS-CoV-2 targets and immune responses, identifying potential therapeutic targets and mechanisms linking IL6 to viral entry.

The paper provides three resources to improve DSF experiments: an interactive theoretical framework, practical tips, and online data analysis tools, available at DSFworld.