Tom P Brown

Unraveling single-molecule reactions via multiplexed in-situ DNA sequencing

This paper introduces SPIN-Seq, a method that links DNA sequence with functional properties at the single-molecule level, revealing sequence-dependent transcription factor interactions and RNA polymerase behavior.

Hierarchical Coordination of Polymerase Theta and RAD51 Resolves Clustered Replication Fork Collapse

The paper shows Pol{theta} is recruited via RAD51 ubiquitylation to resolve clustered ICLs causing replication fork collapse in HR-proficient cells.

Molecular basis for CSB stimulation of the SNM1A DNA repair nuclease

This study identifies how CSB enhances SNM1A nuclease activity during ICL repair by interacting with its nuclease core and altering substrate conformation.

Anchor-Enhanced Bead Design for Reduced Oligonucleotide Synthesis Errors in Single-cell sequencing

The paper introduces an anchor-enhanced bead design to reduce oligonucleotide synthesis errors in single-cell sequencing, improving UMI recovery and feature detection.

Highly accurate barcode and UMI error correction using dual nucleotide dimer blocks allows direct single-cell nanopore transcriptome sequencing

This paper introduces scBUC-seq, a method to correct barcode and UMI errors in single-cell sequencing, enabling direct Nanopore transcriptome sequencing with improved accuracy.

INSIGHT: a population scale COVID-19 testing strategy combining point-of-care diagnosis with centralised high-throughput sequencing

The paper introduces a two-stage COVID-19 testing strategy combining point-of-care diagnosis with centralised high-throughput sequencing to enable population-scale testing.

Dynamics of the 4D genome during lineage specification, differentiation and maturation in vivo

The paper studies chromatin architecture changes during mouse erythroid differentiation using Tiled-C and shows enhancer-promoter interactions form during differentiation, not before gene expression.

Targeted high-resolution chromosome conformation capture at genome-wide scale.

The paper introduces a new method, NuTi Capture-C, that improves high-resolution chromosome conformation capture by reducing noise and increasing sequencing content, enabling reproducible genome-wide interaction profiles.

CRISPRi is not strand-specific and redefines the transcriptional landscape

This paper shows CRISPRi is not strand-specific and alters transcription by enabling both sense and antisense transcript generation.