Oliver Hobert

Genome-engineered C. elegans strains with sex-transformed neurons and gut

The paper describes CRISPR/Cas9-engineered C. elegans strains with tissue-specific sex transformations and validates their effects through gene expression and behavioral analysis.

Terminal selector and subtype selector function across 200 million years of nematode evolution

The paper compares terminal and subtype selectors in two nematode species to study neuronal evolution over 200 million years, finding conservation and novelty in regulatory programs.

Coexistence of phasmid sensory neurons and caudal glands offers a new perspective on cell type evolution in nematodes

The paper shows that phasmid sensory neurons and caudal glands coexist in a nematode, challenging the idea that they evolved from each other during aquatic-to-terrestrial transitions.

Regulatory architecture controlling terminal differentiation of an interoceptive paraneuron in C. elegans

The paper identifies transcriptional programs controlling terminal differentiation of uv1 paraneurons in C. elegans, revealing shared regulatory mechanisms with canonical neurons.

A pilot study for whole proteome tagging in C. elegans

This paper demonstrates a pilot study using fluorescent tags to label 30 C. elegans genes with three fluorophores, revealing new insights into protein expression and localization.

A panoramic view of the expression and function of the Doublesex/DMRT gene family in C. elegans

Reporter alleles of all ten C. elegans DMRT genes show six are sexually dimorphic across tissues; mab-3, dmd-3 and mab-23 nulls alter male neuronal identities.

A conserved C. elegans zinc finger-homeodomain protein, ZFH-2, continuously required for structural integrity and function of alimentary tract and gonad

PHA-4/FoxA controls the function of pharyngeal and extrapharyngeal enteric neurons of C. elegans

This study shows that PHA-4/FoxA is required for the terminal differentiation and lifelong function of multiple enteric neuron types in C. elegans.

A gene expression atlas of a juvenile nervous system

This study creates a gene expression atlas of C. elegans L1 neurons, identifying differentially expressed genes and regulatory networks involved in postembryonic neurogenesis and maturation.

Identity and functions of monoaminergic neurons in the predatory nematode Pristionchus pacificus reveal nervous system conservation and divergence

The paper characterizes monoaminergic neurons in Pristionchus pacificus, identifying conserved and novel patterns of neurotransmitter expression and function in behaviors like host-finding and dispersal.

Gut epithelium modifies enteric behaviors during nutritional adversity via distinct peptidergic signaling axes

The paper shows midgut epithelial cells in C. elegans regulate feeding and defecation behaviors by modulating neuropeptidergic signaling to the enteric nervous system under different nutritional conditions.

Pervasive homeobox gene function in the male-specific nervous system of Caenorhabditis elegans

The paper investigates the role of homeobox genes in defining neuronal diversity in the male-specific nervous system of C. elegans, showing their expression patterns and functional importance.

Comparative connectomics of two distantly related nematode species reveals patterns of nervous system evolution

The paper compares the nervous systems of two nematode species to identify patterns of evolutionary change in their connectomes.

Evolution of lateralized gustation in nematodes

The paper shows that nematodes use left/right asymmetric neurons to process salt cues, revealing evolutionary changes in gustatory systems despite limited neuron numbers.

Molecular patterns of evolutionary changes throughout the whole nervous system of multiple nematode species

The paper analyzes whole-animal single-cell transcriptomes of nematodes to reveal stable neuronal identities but divergent signaling pathways across species.

Nervous system-wide analysis of all C. elegans cadherins reveals neuron-specific functions across multiple anatomical scales

The paper analyzes all C. elegans cadherin genes to uncover their expression patterns and functions in the nervous system, revealing neuron-specific roles across different anatomical scales.

Alternative splicing controls pan-neuronal homeobox gene expression

The paper shows that UNC-75/CELF-mediated alternative splicing controls pan-neuronal expression of the homeobox gene ceh-44/CUX in C. elegans while excluding a Golgi-localized golgin from neurons.

Integrating bulk and single cell RNA-seq refines transcriptomic profiles of individual C. elegans neurons

The paper integrates bulk and single-cell RNA-seq data from C. elegans neurons to improve transcript detection and differential gene analysis.

Functional analysis of conserved C. elegans bHLH family members uncovers lifespan control by a peptidergic hub neuron

The paper identifies the role of C. elegans bHLH genes in specifying neuron identity and function, revealing that hlh-15/NHLH controls lifespan through neuropeptide secretion in AVK neurons.

The mechanosensory DEG/ENaC channel DEGT-1 is a proprioceptor of C. elegans foregut movement

This study identifies DEGT-1 as a mechanosensory ion channel in C. elegans that modulates pharyngeal pumping by detecting proprioceptive feedback from organ movement.

Experience-dependent, sexually dimorphic synaptic connectivity defined by sex-specific cadherin expression

The paper identifies how juvenile experience influences sexually dimorphic synaptic connectivity in C. elegans through sex-specific cadherin expression regulated by CREB and LIN-29.

A neurotransmitter atlas of C.elegans males and hermaphrodites

The paper creates a comprehensive neurotransmitter atlas in C. elegans by analyzing CRISPR/Cas9 knock-in reporter strains, revealing new expression sites and co-expression patterns.

Comparative analysis of new, mScarlet-based red fluorescent tags in Caenorhabditis elegans

The paper compares the brightness and maturation time of new red fluorescent tags in C. elegans and finds mScarlet-I3 outperforms older versions and is comparable to GFP.

Toolkits for detailed and high-throughput interrogation of synapses in C. elegans

The paper introduces WormPsyQi, an image analysis pipeline for high-throughput quantification of synaptically localized fluorescent signals, along with 30 transgenic C. elegans strains for synaptic labeling.

Restricted effects of the sole C. elegans Daughterless/E homolog, HLH-2, on nervous system development

This study investigates the role of hlh-2, the C. elegans homolog of Daughterless/E, in neurogenesis and finds that its effects are restricted rather than universal across different neuron lineages.

Neuronal contact predicts connectivity in the C. elegans brain

The paper evaluates C. elegans connectomes to assess whether neuronal adjacency predicts synaptic connectivity, supporting Peters rule over the lock-and-key model.

The neuropeptidergic connectome of C. elegans

The paper generates a draft connectome of neuropeptide signaling in C. elegans by integrating single-cell data with biochemical analysis.

Widespread employment of conserved C. elegans homeobox genes in neuronal identity specification

The paper investigates the role of homeobox genes in neuronal identity specification in C. elegans, finding that 111 of 118 neuron classes require homeobox genes for proper differentiation.

C. elegans Sine oculis/SIX-type homeobox genes act as homeotic switches to define neuronal subtype identities

The paper shows that homeobox genes control neuronal subtype identities in C. elegans by acting as toggle switches to differentiate between closely related neuron classes.

Integrating bulk and single cell RNA-seq refines transcriptomic profiles of individual C. elegans neurons

The paper integrates bulk and single-cell RNA-seq data from C. elegans neurons to improve transcript detection and differential gene analysis.