Karsten Weis

This study shows PKA regulates cytoplasmic density by controlling glycogen and trehalose, which affect diffusion and confer stress resistance.

The paper introduces a method to pulse-label yeast Nuclear Pore Complexes using a nanobody, enabling tracking in imaging and biochemical experiments.

This study identifies Jjj2 as a chaperone that regulates nucleoporin condensation in budding yeast, showing its role in maintaining NPC permeability and preventing aggregation.

This study uses Scanning Ion Conductance Microscopy to measure the material properties of biomolecular condensates, showing how pH and protein tags affect their stiffness over time.

This study identifies Brl1 and Brr6 as conserved fusogens mediating membrane fusion during NPC assembly in eukaryotes.

The paper introduces in-cell LiP-MS to study protein structural changes and biomolecular condensates in live human cells, revealing structural alterations under stress and validating them with microscopy.

The paper uses solution scattering and simulations to show that Dhh1's modular architecture allows structural heterogeneity while maintaining functional specificity.

The paper shows that during acute glucose withdrawal in yeast, newly synthesized stress mRNAs escape translational repression while pre-existing mRNAs are repressed, revealing a timing-based coordination of transcriptional and translational responses.

This study shows that respiration-induced gene expression and stress response pathways are critical for maintaining cytoplasmic properties and survival in yeast quiescence.

This study shows that polysomes and mRNA organization control cytoplasmic biophysical properties by affecting diffusivity through translation inhibition and mRNA condensation.

This paper introduces a nanobody-based method to detect newly forming nuclear pore complexes in yeast, revealing novel insights into their assembly and localization.

This study shows that fluorescent and poly-histidine tags alter the condensation properties of Dhh1 in vitro and P-body numbers in vivo, raising concerns about the reliability of tagged protein condensation experiments.

This study shows that VMP1 and TMEM41b are required for primitive endoderm specification by regulating WNT signaling in mouse ESCs.

The paper characterizes RNA content in individual phase-separated coacervate microdroplets using high-throughput sequencing and identifies sequence motifs associated with RNA accumulation.

The paper identifies bulk autophagy and β-oxidation as critical energy providers during acute glucose starvation in yeast.

The paper measures mRNA decay in yeast and finds translation initiation, not codon optimality or elongation, determines mRNA stability, with an average half-life of 4.8 minutes.