Stuart S. Levine

The paper develops scalable cell-free protein synthesis platforms to generate single-cell RNA-sequencing data on immune cell responses to cytokines and other signals, enabling functional discovery and drug target prioritization.

This paper uses a hybrid capture method to profile temporal gene expression in Rickettsia during early infection, identifying gene clusters and antisense RNAs.

The paper assesses the mutagenic impact of fluorescent dyes during NGS library preparation and finds minimal effects on mutation rates and mutational signatures.

This study identifies persistent interferon signaling and clonal expansion as early events in NDMA-induced liver cancer by analyzing molecular changes in mice.

This study shows CFDP1 is required for H2A.Z deposition by the SRCAP chromatin remodeling complex and links CFDP1 deficiency to reduced histone modifications and developmental gene upregulation.

This study shows that CDK9 degradation with KI-CDK9d-32 is more effective than inhibition in disrupting MYC transcriptional networks and causes MYC downregulation and nucleolar destabilization.

This study evaluates the functional impact of genetic variants using a high-throughput prime editing sensor strategy, identifying both known and novel TP53 variants with diverse effects on p53 function.

The paper introduces AQRNA-seq, a method for accurate small RNA quantification, and demonstrates its application to bacterial tRNA pools revealing copy number variation and modification patterns.

This study uses single-cell transcriptomics to analyze aged mouse brains, identifying cell-type-specific aging signatures and highlighting molecular processes like ribosomal biogenesis.

The paper investigates how iron, heme, and HRI regulate protein translation and gene expression in erythroblasts during iron deficiency, identifying novel pathways and regulators of erythropoiesis.

The paper compares ribosomal depletion kits for RNAseq library construction, finding differences in ease of use and transcript length impact on library efficiency.