Vincent L. Butty

This paper develops CRISPR-MACE, a platform for continuous directed evolution of CRISPR-Cas9 in human cells, generating variants with enhanced DNA binding and resistance to AcrIIA4.

This paper uses a hybrid capture method to profile temporal gene expression in Rickettsia during early infection, identifying gene clusters and antisense RNAs.

The paper assesses the mutagenic impact of fluorescent dyes during NGS library preparation and finds minimal effects on mutation rates and mutational signatures.

This study examines how the duration of initial HIV-1 viremia affects the functional properties of HIV-specific T cell receptors in individuals with HLA-B*58:01.

The paper shows that HSF1 activation supports the emergence of cancer-associated p53 mutations by enhancing their fitness under nutlin-3 pressure.

This paper develops a high-throughput optogenetic assay for skeletal muscle exercise studies, revealing transcriptional responses to optical stimulation and hypoxia.

This study shows CFDP1 is required for H2A.Z deposition by the SRCAP chromatin remodeling complex and links CFDP1 deficiency to reduced histone modifications and developmental gene upregulation.

This study uses iPSC-derived cartilage to show that the Arg719Cys COL2A1 mutation causes defective proteostasis, leading to abnormal matrix deposition and disease.

This study uses 2.5D actuating substrates to show that both mechanical and biochemical effects of muscle exercise significantly influence motor neuron growth and maturation.

The paper develops an iPSC-derived cartilage model to study COL2A1 mutations, revealing that Arg719Cys substitution causes defective collagen-II folding and secretion, leading to matrix dysfunction and osteoarthritis.

This study identifies RBPMS2 as a conserved regulator of alternative splicing that supports myofibril organization and calcium handling in cardiomyocytes across species.

The paper uses single cell RNA-seq to study human hematopoietic stem and progenitor cells (HSPCs) across development and aging, revealing age-related transcriptional changes and differentiation trajectories.

This study uses single-cell transcriptomics to analyze aged mouse brains, identifying cell-type-specific aging signatures and highlighting molecular processes like ribosomal biogenesis.

The paper investigates how iron, heme, and HRI regulate protein translation and gene expression in erythroblasts during iron deficiency, identifying novel pathways and regulators of erythropoiesis.

The paper compares ribosomal depletion kits for RNAseq library construction, finding differences in ease of use and transcript length impact on library efficiency.