Lee L. Rubin

Combinatorial Modulation of Wnt, STAT3, TGF-β, and Tie2 Pathways Drives Brain Endothelial Cell-Like Differentiation from hiPSCs

The paper identifies signaling pathways that drive the differentiation of brain endothelial cells from hiPSCs in vitro.

Isogenic cortical organoids enable precision targeting of APP variant-specific pathways in Alzheimer's disease

The paper uses isogenic cortical organoids to identify variant-specific pathogenic pathways in Alzheimer's disease and demonstrates targeted interventions that restore neuronal survival.

Microbiome depletion rejuvenates the aging brain

The paper shows that depleting the gut microbiome in aged mice rejuvenates the brain by improving vascular density, myelination, neurogenesis, and reducing inflammation.

An induced pluripotent stem cell-based chemical genetic approach for studying spinal muscular atrophy

The paper uses a chemical genetic approach with induced pluripotent stem cells to screen for compounds affecting SMA neuronal gene expression, generating a queryable dataset of transcriptional profiles.

A human iPSC-derived motor neuron-myogenic cell coculture platform to evaluate neuromuscular junction innervation after axon injury and in Spinal Muscular Atrophy

This paper develops a human iPSC-derived motor neuron-myogenic coculture platform to study NMJ innervation after axon injury and in SMA, showing blebbistatin enhances NMJ reinnervation and confirming reduced NMJ function in SMA patient-derived neurons.

Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells

This study develops a rapid, scalable method to generate functional human astrocytes from pluripotent stem cells using NGN2 induction, showing high homogeneity and disease relevance.

Coupling comprehensive pesticide-wide association study to iPSC dopaminergic screening identifies and classifies Parkinson-relevant pesticides

The paper combines epidemiological pesticide exposure data with iPSC-derived dopaminergic neuron screening to identify and classify pesticides linked to Parkinson's disease.

An automated high-content synaptic phenotyping platform in human neurons and astrocytes reveals a role for BET proteins in synapse assembly

The paper develops an automated high-content synaptic phenotyping platform in human neurons and astrocytes, identifying BET inhibitors as key regulators of synapse assembly.

Gasdermin-E mediates mitochondrial damage in axons and neurodegeneration

This study identifies GSDME as a mediator of mitochondrial damage and axon loss in neurodegenerative diseases, showing its role in ALS and FTD models.

Heterochronic parabiosis reprograms the mouse brain transcriptome by shifting aging signatures in multiple cell types

This study uses single-cell transcriptomics to show that heterochronic parabiosis alters aging signatures in multiple brain cell types, with endothelial cells showing significant transcriptional changes.

Fezf2 transient expression via modRNA with concurrent SIRT1 inhibition enhances differentiation of cortical subcerebral / corticospinal neuron identity from mES cells

The paper shows that inhibiting SIRT1 with modRNA-enhanced Fezf2 expression improves SCPN/corticospinal neuron differentiation from mES cells.

Single-cell transcriptomics of the aged mouse brain reveals convergent, divergent and unique aging signatures

This study uses single-cell transcriptomics to analyze aged mouse brains, identifying cell-type-specific aging signatures and highlighting molecular processes like ribosomal biogenesis.