Tom Charlesworth

Advancing Neurological Liquid Biopsy via Six-Base Sequencing of Cerebrospinal Fluid Cell-Free DNA

This paper uses 6-base sequencing to analyze DNA modifications in cerebrospinal fluid cell-free DNA, providing insights into neurodegenerative biology for liquid biopsy applications.

5-methylcytosine and 5-hydroxymethylcytosine are synergistic biomarkers for early detection of colorectal cancer

This study shows that combining 5mC and 5hmC measurements improves early CRC detection accuracy compared to conflated markers.

Temporally discordant chromatin accessibility and DNA demethylation define short and long-term enhancer regulation during cell fate specification

The paper shows that chromatin accessibility and DNA demethylation occur at different times during enhancer regulation, with methylation states predicting chromatin dynamics using machine learning.

Accurate simultaneous sequencing of genetic and epigenetic bases in DNA

This paper introduces a method to simultaneously sequence genetic and epigenetic bases in DNA with single-base resolution, demonstrating accuracy and versatility in human genomic and cell-free DNA samples.