Sina Ghaemmaghami

Mass spectrometric analysis of free methionine oxidation levels in E. coli

This study introduces fMObB, a mass spectrometry method to measure free methionine oxidation in E. coli, revealing higher oxidation under stress and the role of methionine sulfoxide reductases.

Long-lived mammals contain more phosphorylation sites in the SIRT6 C-terminus that enhance PARP1 interaction and resistance to oxidative stress

This study identifies phosphorylation sites in the SIRT6 C-terminus that enhance PARP1 interaction and oxidative stress resistance, correlating with maximum lifespan in mammals.

Protein entanglement misfolding determines divergent fates: proteasomal degradation or persistence in near-native misfolded states

The paper investigates how protein entanglement misfolding affects proteasomal degradation in human cells, finding that entangled proteins are more likely to be ubiquitinated and degraded.

Catabolism of extracellular glutathione supplies amino acids to support tumor growth

This study shows that extracellular glutathione supports tumor growth by providing amino acids through catabolism, and inhibiting this process slows tumor growth.

Structural proteomics of the human ubiquitinome

This study uses structural proteomics to show that ubiquitination patterns differ between misfolded nascent proteins and mature proteins, influencing their degradation rates.

Pervasive divergence in protein thermostability is mediated by both structural changes and cellular environments

This study shows that both structural changes and cellular environments contribute to differences in protein thermostability between two yeast species.

Temporal Single Cell Analysis of Leukemia Microenvironment Identifies Taurine-Taurine Transporter Axis as a Key Regulator of Myeloid Leukemia

This study identifies the taurine-taurine transporter axis as a critical regulator of myeloid leukemia using temporal single-cell RNA-sequencing and functional experiments.

Antibiotic treatment modulates Escherichia coli-derived bacterial extracellular vesicle (BEV) production and their capacity to upregulate ICAM-1 in human endothelial cells

This study shows antibiotic treatment affects BEV production and their ability to upregulate ICAM-1 in endothelial cells, with meropenem increasing BEV production and stimulatory capacity in E. coli strains.

O-GlcNAc modifications regulate lamin A tail processing

This study investigates how O-GlcNAc modifications influence lamin A tail processing and identifies a potential link between glucose metabolism and lamina biogenesis.

Enzymatic synthesis of isotopically labeled hydrogen peroxide for mass spectrometry-based applications

The paper describes a method to enzymatically synthesize isotopically labeled hydrogen peroxide (H218O2) for use in mass spectrometry to accurately measure methionine oxidation levels.

Formylation facilitates the reduction of oxidized initiator methionines

This study shows that formylation of initiator methionines enhances their enzymatic reduction and mitigates oxidation-related misprocessing.

Turnover and replication analysis by isotope labeling (TRAIL) reveals the influence of tissue context on protein and organelle lifetimes

TRAIL quantifies protein and cell lifetimes across tissues, showing cell division drives non-selective turnover in proliferative tissues while physicochemical features influence turnover in non-proliferative tissues.

Interspecies differences in proteome turnover kinetics are correlated with lifespans and energetic demands

The paper measures protein turnover rates in fibroblasts from long-lived species and finds slower turnover correlates with longer lifespan and reduced ROS.