Francisco Javier García-Rodríguez

Single-cell metabolomics reveals infection-specific metabolic reprogramming of human macrophages

This study uses single-cell metabolomics to show that Legionella pneumophila infection induces metabolic reprogramming in human macrophages, with bacterial effectors promoting glycolytic shifts.

Provision of Preferred Nutrients to Macrophages Enables Salmonella to Replicate Intracellularly Without Relying on Type III Secretion Systems

This study shows Salmonella can replicate in macrophages without Type III Secretion Systems if provided with preferred nutrients, highlighting the role of host metabolic heterogeneity in bacterial replication.

The Legionella effector RidL promotes mitochondrial fragmentation through phosphorylation activation of the large GTPase Drp1

This study identifies RidL as a bifunctional effector that inhibits retrograde trafficking and promotes mitochondrial fragmentation by phosphorylating Drp1, aiding Legionella pneumophila's intracellular survival.

Complex-I Preserves Mitochondrial Polarization during Infection of Human Macrophages by Secretion-competent Bacteria

This study identifies Complex I as essential for maintaining mitochondrial membrane potential during infection with virulent bacteria, revealing distinct strategies used by Legionella and Salmonella.

Backtracking Metabolic Dynamics in Single Cells Predicts Bacterial Replication in Human Macrophages

The paper tracks metabolic dynamics in single human macrophages infected with Legionella pneumophila to predict bacterial replication with 83% accuracy using machine learning.

SifA-mediated Remodeling of the Salmonella-Containing Vacuole Prevents Bacterial Dormancy by Promoting Nutrient Accessibility

This study identifies SifA as a key effector that prevents Salmonella dormancy by promoting nutrient accessibility through SIF-mediated remodeling of the Salmonella-containing vacuole.

2-deoxyglucose transiently inhibits yeast AMPK signaling and triggers glucose transporter endocytosis, potentiating the drug toxicity

This paper investigates how 2-deoxyglucose (2DG) induces endocytosis of plasma membrane proteins in yeast, revealing a role for the Snf1/AMPK pathway and Rod1 in mediating 2DG resistance.