Juha T. Huiskonen

In situ Structure of the Human Gap Junction

The paper determines the in situ structure of human Cx43 gap junction plaques using cryogenic electron tomography, revealing the role of the C-terminal domain in plaque assembly.

AAV2 Crosslinks Actin Filaments: Implications for AAV Gene Therapy Vector Design

This study identifies AAV2's ability to bundle actin filaments, suggesting a new class of actin-binding viruses and offering insights for improving AAV gene therapy.

Gating Mechanism of the Human Connexin 45 Gap Junction Channel

This study determines the structural basis of Cx45 gating and Ca2+ regulation using cryo-EM, revealing a dual steric-electrostatic mechanism.

Capsid Restructuring Activates Semi-Conservative dsRNA Transcription in Cystovirus Φ6

This study visualizes the activation of semi-conservative dsRNA transcription in cystovirus Φ6 by observing capsid restructuring and transcription complex assembly using cryogenic electron microscopy.

Structural characterization of antibody-responses from Zolgensma treatment provides the blueprint for the engineering of an AAV capsid suitable for redosing

This study uses cryo-EM to analyze antibodies against AAV9 vectors, identifying residues that enable capsid engineering to avoid neutralization and allow redosing.

Folding of mRNA-DNA origami for controlled translation and viral vector packaging

This study investigates how design parameters affect mRNA-DNA origami folding and translation efficiency, showing the importance of ribosome-binding sequences and demonstrating their encapsulation in virus capsids for enhanced delivery.