Benjamin G. Gowen

Cis-Regulatory Element and Transcription Factor Circuitry Required for Cell-Type Specific Expression of FOXP3

The paper identifies cis-regulatory elements and transcription factors controlling FOXP3 expression in human Tregs and Tconvs, revealing mechanisms that restrict FOXP3 to Tregs in mice.

Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines

This study evaluates subretinal delivery of Cas9 ribonucleoproteins in mice and pigs, showing variable editing efficiency and highlighting species-specific challenges for therapeutic translation.

ATF4 mediates fetal globin upregulation in response to reduced β-globin

The paper identifies ATF4 as a key regulator of fetal globin re-expression in response to reduced β-globin, providing mechanistic insight into stress-induced globin compensation.

Enhancer connectome in primary human cells reveals target genes of disease-associated DNA elements

This paper uses HiChIP to map enhancer-promoter interactions in primary human cells, linking disease-associated DNA variants to their target genes.

Discovery of an autoimmunity-associated IL2RA enhancer by unbiased targeting of transcriptional activation

The paper identifies an IL2RA enhancer associated with autoimmunity using CRISPRa and shows it affects gene activation timing without blocking expression.