Pascal S. Kaeser

This paper engineers proteins to restore and enhance neurotransmitter release by reconstituting active zone functions in neurons.

This study investigates the role of Munc13 in evoked somatodendritic dopamine release in dopamine neurons, finding it essential for evoked but not spontaneous release.

This study shows that endocytic protein localization to periactive zones in synapses is independent of active zone assembly and synaptic activity.

The paper identifies enhancer-AAV tools for specific neuronal subtypes and validates them across species.

The paper shows that high innervation density of cross-reacting transmitters causes non-specific activation of GPCR-based sensors, requiring specific innervation silencing for accurate interpretation.

The paper identifies the C-terminus of voltage-gated Ca2+ channels as the determinant for their compartment-specific targeting in neurons.

The paper identifies distinct molecular machineries for Ca2+ channel clustering and synaptic vesicle priming at active zones, showing that Liprin- and PTPσ specifically support priming site assembly.

The paper identifies the protein composition of dopamine release sites in the striatum using proximity proteomics and shows that RIM organizes these sites.

The paper investigates whether Munc13 supports vesicle fusogenicity independently of active zone scaffolds and synaptic vesicle docking.

The paper investigates active zone functions by knocking out RIM+ELKS and reconstituting key processes, showing that docking and fusion competence are separable and scaffolding is not required for release.

The paper identifies key proteins and domains involved in dopamine release in the striatum, showing that RIM, Munc13, and Liprin- are essential while other active zone proteins are not.

This study shows that post-tetanic potentiation (PTP) persists in mice with Munc18-1 unable to be phosphorylated by PKC, indicating PTP mechanisms are independent of this phosphorylation.

This study shows phosphorylation of Liprin-3 triggers phase separation to regulate presynaptic active zone structure and function.

This study investigates the role of LAR-RPTPs in synapse structure and function by analyzing triple knockout mice, finding they are dispensable for presynaptic organization and function.

The paper shows that firing rate homeostasis in cultured mouse neurons occurs without activity-regulated transcription, challenging the hypothesis that transcription is required for this process.