Cosmin Saveanu

A large-scale analysis of the R2TP chaperone network reveals its contribution to the assembly of INO80, SRCAP and TIP60

The paper identifies RPAP3 and PIH1D1 as R2TP subunits and shows their role in assembling INO80, SRCAP, and TIP60 complexes through physical interactions and functional validation.

Most Upf1-associated mRNAs have short poly(A) tails, lack a premature termination codon and are targeted by NMD.

The paper shows that most Upf1-associated mRNAs have short poly(A) tails, lack PTCs, and are targeted by NMD, redefining the pathway's scope.

Mapping interactions within the NMD decapping complex reveals how Upf1 recruits Dcp2 onto mRNA targets

The paper maps interactions within the NMD decapping complex, showing how Upf1 recruits Dcp2 onto mRNA targets through domain-specific binding and competition.

Identification of an evolutionary conserved binding motif responsible for the recruitment of NMD factors to the UPF1 helicase

The paper identifies a conserved binding motif in Nmd4 that interacts with Upf1, showing its role in NMD and conservation in metazoan SMG6.

Deadenylation rate is not a major determinant of RNA degradation in yeast

The paper shows that mRNA stability in yeast is determined by decapping speed, not deadenylation rate, with unstable mRNAs having rapid decapping and longer poly(A) tails.

Investigation of RNA metabolism through large-scale genetic interaction profiling in yeast

The paper performs large-scale genetic interaction screens in yeast to study RNA metabolism, identifying gene links to biological processes and validating a specific connection to mRNA translation initiation.

Detection and Degradation of Nonsense-mediated mRNA Decay Substrates Involve Two Distinct Upf1-bound Complexes

The paper identifies two distinct Upf1-bound complexes in yeast NMD, Detector and Effector, and shows their roles in substrate detection and degradation.

Actin activates Pseudomonas aeruginosa ExoY nucleotidyl cyclase toxin and ExoY-like effector domains from MARTX toxins

The paper shows that actin activates ExoY and ExoY-like cyclases from pathogens, revealing a new class of actin-activated nucleotidyl cyclase toxins.