Christopher L. Holley

snoCLASH Reveals Extensive snoRNA-mRNA Interaction Networks

The paper introduces snoCLASH, a method to identify snoRNA-mRNA interactions, revealing thousands of interactions and validating a target with functional effects on protein expression.

Genome-wide profiling of RNA 2'-O-methylation in neurons and identification of orphan snoRNA targets

The paper compares RNA 2'-O-methylation patterns in neurons with and without specific snoRNA deletions, identifying new targets of orphan snoRNAs.

Rpl13a snoRNAs Downregulate Smooth Muscle Cell COX4I2 and Promote Neointimal Hyperplasia

This study shows that Rpl13a snoRNAs increase SMC ROS levels by reducing COX4I2 expression, leading to neointimal hyperplasia.

Generating snoRNA-guided Programmable 2'O-methylation

This paper introduces an RNase H-based assay to validate 2'O-methylation sites and demonstrates synthetic snoRNAs can guide Nm modifications in mRNA.

Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique

The paper introduces PA-{Psi}-seq, a technique to map pseudouridine residues on cellular and viral transcripts, and shows that HIV-1 transcripts are not affected by PUS enzyme inactivation.

Altered m6A modification of specific cellular transcripts affects Flaviviridae infection

This study shows that Flaviviridae viruses alter m6A modification of specific host transcripts, affecting viral infection through changes in translation and splicing.