Author

Gianlucca Gonçalves Nicastro

National Institutes of HealthORCID 0000-0002-3133-2441Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:statistical model· 4 Organism:bacteria (other)· 2 Research Focus:cell signaling & trafficking· 2 Approach:experimental assay· 2 Research Focus:host-pathogen & immune response· 2 Organism:multiple species· 2 Assay:proteomics· 2 Research Focus:cell type annotation· 1

Frequent Co-Authors

Lakshminarayanan AravindiD· 5 shared Ethel Bayer‐SantosiD· 3 shared Julia Takuno HespanholiD· 2 shared Kristi Pham· 2 shared Robson Francisco de SouzaiD· 2 shared Akanksha VarshneyiD· 1 shared Alexander Maxwell BurroughsiD· 1 shared Arlen JohnsoniD· 1 shared

Activity by Year

2025-2026 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

5 shared papersMicrobiology

Lakshminarayanan AravindORCID

Mutual co-authors in cluster

Author profile →

5 papers

Recent·Most cited

An RNase Toxin Hijacks Elongation Factor-Tu to Cleave the Ribosomal Sarcin-Ricin Loop

Julia Takuno Hespanhol, Taylor M Blackburn, Kristi Pham, Akanksha Varshney, Gianlucca Gonçalves Nicastro, Robson F. de Souza, Lakshminarayanan Aravind, Arlen Johnson, Kenneth C. Keiler, Christine M. Dunham, Ethel Bayer‐Santos

This paper characterizes SRLase1, an RNase toxin that cleaves the sarcin-ricin loop of 23S rRNA by repurposing EF-Tu, revealing a new mechanism of ribosome inhibition.

bioRxiv · Sep 19, 2026 · PreprintMicrobiology

A structure-guided classification framework reveals the diversity and catalytic architecture of BECR ribonuclease

Kristi Pham, Gianlucca Gonçalves Nicastro, Audrey R Long, Lakshminarayanan Aravind, Claus O. Wilke, Robson Francisco de Souza, Ethel Bayer‐Santos

The paper classifies BECR ribonuclease proteins across domains of life using structural and genomic analysis, revealing new families and refining existing classifications.

bioRxiv · Aug 29, 2026 · PreprintMicrobiology

Genome-directed study reveals the diversity of Salmonella T6SS effectors and identifies a novel family of lipid-targeting antibacterial toxins

Gianlucca Gonçalves Nicastro, Stephanie Sibinelli‐Sousa, Julia Takuno Hespanhol, Thomas W. C. Santos, Joseph P. Munoz, Rosangela S. Santos, Blanca M. Perez-Sepulveda, Sayuri Miyamoto, L. Aravind, Robson Francisco de Souza, Ethel Bayer‐Santos

The paper identifies a novel family of lipid-targeting antibacterial toxins from Salmonella using in-silico analysis of genomes and biochemical characterization.

PLoS Biology · Mar 17, 2026Peer-reviewedMicrobiology

A methylome-derived m6-dAMP trigger assembles a PUA-Cal-HAD immune filament that depletes dNTPs to abort phage infection

Zhiying Zhang, Yi Wu, Yan‐Jiun Lee, Gianlucca Gonçalves Nicastro, Arpita Chakravarti, Hui Fang, D. Taverner, Peter Weigele, Lakshminarayanan Aravind, Dinshaw J. Patel, Franklin L. Nóbrega

The paper identifies a methylome-derived m6-dAMP trigger that forms an immune filament in E. coli to deplete dNTPs and abort phage infection.

bioRxiv · Jan 16, 2026 · PreprintMicrobiology

The Lipocone Superfamily: A Unifying Theme In Metabolism Of Lipids, Peptidoglycan And Exopolysaccharides, Inter-Organismal Conflicts And Immunity

Alexander Maxwell Burroughs, Gianlucca Gonçalves Nicastro, Lakshminarayanan Aravind

The paper identifies the Lipocone superfamily as a unifying theme in lipid metabolism, inter-organismal conflicts, and immunity across species.

eLife · Sep 9, 2025Peer-reviewedBioinformatics