Polyethylene glycol in human biofluids can mark drug exposure rather than contamination; it is oxidized to acid metabolites and appears in 2.3% of public metabolomics files.

bioRxiv · Sep 18, 2026 · PreprintBiochemistry✦ In-depth digest

Plate-based ISD-SPE enables dual proteome-secretome concentration-response profiling of TLR signalling in iPSC-derived macrophages

Chloe L. Tayler, Mengchun Li, Kerena Norris, Lee M. Booty, Rebecca Beveridge, Nicholas J. W. Rattray, Rachel E. Heap

This paper introduces a plate-based ISD-SPE workflow for integrated proteome and secretome analysis of TLR signaling in macrophages, showing improved reproducibility and scalability for drug discovery.

bioRxiv · Jul 21, 2026 · PreprintImmunology

dia-PASEF Enables Rapid Profiling of the Human Secretome for Deeper Insights into Cellular Dynamics and Inflammatory Mechanisms

Chloe L. Tayler, Serena Bateman, Charlie Haslam, Leonie Müller, Kerena Norris, Evan Rosa-Roseberry, Stan Martens, Jonathan Yu, Eleanor R. Dickinson, Lee M. Booty, Rebecca Beveridge, Nicholas J. W. Rattray, Rachel E. Heap

The paper introduces dia-PASEF, a mass spectrometry workflow for rapid secretome profiling, revealing novel inflammatory responses and dynamic secretion patterns in macrophages.

Molecular & Cellular Proteomics · May 28, 2026Peer-reviewedImmunology

Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue

Kristina Tomkova, Jonathan W. Lewis, Thomas A. Nicholson, Animesh Acharjee, Aidan Anderson, Jonathan P. Barlow, Kathryn Frost, Thomas Jackson, João Pedro Magalhaes, Sudip Mondal, Amy J. Naylor, Aline Nixon, Nicholas J. W. Rattray, Paula Rudzinska, Amanda Veiga Sardeli, , Helen Michelle McGettrick

The paper develops a toolkit to measure multiple ageing hallmarks in human blood and tissue samples, identifying age-related changes in immune cells, senescence, and mitochondrial function.

bioRxiv · Apr 22, 2026 · PreprintImmunology

Pan-repository analysis reveals a drug-activating function of microbial bile acid conjugation

Vincent Charron‐Lamoureux, Patricia E. Kelly, Simone Zuffa, Abubaker Patan, Marta Sala‐Climent, Corinn Walker, Haoqi Nina Zhao, Shipei Xing, Harsha Gouda, Julius Agongo, Erin R. Reilly, Lina Sallam, Selene F. H. Shore, Subhomitra Ghoshal, Anika K Harpavat, , Pieter C. Dorrestein

The paper identifies microbial bile acid conjugation as a drug-activating function for 5-ASA, revealing its therapeutic relevance through metabolomics analysis.

bioRxiv · Mar 4, 2026 · PreprintBiochemistry

OMICmAge: An integrative multi-omics approach to quantify biological age with electronic medical records

Qingwen Chen, Varun Bhamidipati Dwaraka, Natàlia Carreras-Gallo, Kevin M. Mendez, Yulu Chen, Sofina Begum, Priyadarshini Kachroo, Nicole Prince, Hannah Went, Tavis L. Mendez, Aaron Lin, Logan Turner, Mahdi Moqri, Su H. Chu, Rachel S. Kelly, , Jessica Ann Lasky-Su

The paper develops OMICmAge, a multi-omics biomarker of biological age, and shows it outperforms existing markers in predicting chronic diseases and mortality.

bioRxiv · Oct 16, 2023 · PreprintBioinformatics

Integration of mass-spectrometry-based metabolomics and proteomics to characterise different senescence induced molecular sub-phenotypes

Domenica Berardi, Gillian Farrell, A. Al-Sultan, A McCulloch, Zahra Rattray, Nicholas J. W. Rattray

The paper integrates mass-spectrometry-based metabolomics and proteomics to identify molecular sub-phenotypes of senescence induced by different stressors.

bioRxiv · Nov 30, 2022 · PreprintMolecular Biology