Léo Guignard

A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

The paper develops a pipeline for deep imaging and analysis of multi-layered organoids, revealing gene expression patterns and nuclear morphology in gastruloids.

Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy

This paper accelerates fluorescence lifetime imaging (FLIM) using single-objective light-sheet microscopy, achieving faster and less phototoxic imaging of live multicellular systems.

Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

The paper introduces Track2p, a cell-tracking method for longitudinal analysis of neuronal activity in developing mouse brains, revealing a developmental transition in activity statistics.

Epigenetic priming of embryonic lineages in the mammalian epiblast

The paper identifies epigenetic priming of lineage-specific fates in the mammalian epiblast, revealing early specification of endothelial and mesodermal lineages.

Automated Reconstruction of Whole-Embryo Cell Lineages by Learning from Sparse Annotations

This paper introduces a method for automated lineage reconstruction in developing embryos using sparse annotations, achieving 75.8% accuracy on mouse embryos compared to 31.8% previously.

Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

The paper describes a method to generate trunk-like structures from mouse embryonic stem cells that mimic embryonic development features.

Contact-dependent cell communications drive morphological invariance during ascidian embryogenesis

The paper investigates how contact-dependent cell communications maintain morphological invariance during ascidian embryogenesis despite genomic divergence.

Evolution of the embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians.

This study investigates the evolution of cis-regulatory elements in Phallusia and Ciona ascidians, finding conserved regulatory logic and enhancer structures despite genomic divergence.

Reconstruction of cell lineages and behaviors underlying arthropod limb outgrowth with multi-view light-sheet imaging and tracking

The paper uses multi-view light-sheet imaging and a new tracking tool to reconstruct cell lineages and behaviors during crustacean limb development, revealing how cell proliferation and division orientation drive limb outgrowth.