Lotta Happonen

Multi-Enzyme Data-Independent Acquisition Mass Spectrometry for Proteoform-Aware Single-shot Proteomics

The paper introduces mE-DIA, a multi-enzyme DIA-MS workflow that improves proteoform detection in proteomics by integrating analysis and interpretation into a single toolset.

Resistance to antibacterial peptide nucleic acids through altered ribosome function

This study identifies a ribosome-centered mechanism of resistance to antibacterial PNAs in E. coli, showing that mutations in ribosomal protein uS12 alter 30S assembly and translation-initiation dynamics, reducing PNA efficacy.

Structural profiling of the pneumolysin epitope landscape uncovers a cross-species neutralising site across cholesterol-dependent cytolysins

The paper maps the structural epitope landscape of pneumolysin and identifies a conserved cross-species neutralising site among cholesterol-dependent cytolysins.

Streptococcus pyogenes nuclease A interferes with host complement functions

This study identifies SpnA's role in inhibiting the complement system by interacting with C5b67, preventing membrane attack complex assembly.

Design of a Streptolysin O Epitope-Centric Nanoparticle Vaccine Against Streptococcus pyogenes

This paper designs an epitope-centric nanoparticle vaccine against Streptococcus pyogenes using structural mass spectrometry and deep learning to enhance immunogenicity.

CDNF rescues human iPSCs-derived dopamine neurons through direct binding to unfolded protein response sensors PERK and IRE1α

This study identifies CDNF's mechanism of action by showing it rescues human dopamine neurons through direct binding to UPR sensors PERK and IRE1α.

Streptolysin O Accelerates the Conversion of Plasminogen to Plasmin

This study shows that streptolysin O enhances plasminogen to plasmin conversion by interacting with plasminogen and altering its conformation to increase sensitivity to proteolytic processing.

Multienzyme deep learning models improve peptide de novo sequencing by mass spectrometry proteomics

This study develops multienzyme deep learning models for de novo peptide sequencing from mass spectrometry data, showing improved generalizability and sequence coverage across diverse protease digests and species.

Structural determination of Streptococcus pyogenes M1 protein interactions with human immunoglobulin G using integrative structural biology

This study determines the structural interactions between Streptococcus pyogenes M1 protein and human IgG using integrative structural biology, revealing conserved mechanisms to inhibit phagocytosis.