The paper investigates the distinct roles of PRC2 subcomplexes in epigenetic repression and stem cell differentiation using human pluripotent stem cells.

bioRxiv · May 15, 2025 · PreprintMolecular Biology

Discovery and characterization of LNCSOX17 as an essential regulator in human endoderm formation

Alexandro Landshammer, Adriano Bolondi, Helene Kretzmer, Christian Much, René Buschow, Alina Rose, Hua‐Jun Wu, Sebastian D. Mackowiak, Bjoern Braendl, Pay Gießelmann, Rosaria Tornisiello, Krishna Mohan Parsi, Jack D. Huey, Thorsten Mielke, David Meierhofer, , Alexander Meissner

The paper identifies and characterizes LNCSOX17, a lncRNA essential for human endoderm formation, showing its role in regulating transcriptome and differentiation.

eLife · Jan 16, 2023Peer-reviewedGenomics

The temporal dynamics of lncRNA Firre-mediated epigenetic and transcriptional regulation

Christian Much, Erika L. Lasda, Isabela Tiemy Pereira, Tenaya K. Vallery, Daniel Ramirez, Jordan P. Lewandowski, Robin D. Dowell, Michael J. Smallegan, John L. Rinn

This study investigates the temporal dynamics of lncRNA Firre-mediated epigenetic and transcriptional regulation, showing rapid and persistent effects on gene expression.

bioRxiv · May 15, 2022 · PreprintGenomics

Molecular basis of indispensable accuracy of mammalian miRNA biogenesis

David Zapletal, Eliška Táborská, Josef Pasulka, Radek Malı́k, Karel Kubíček, Martina Zanova, Christian Much, Marek Šebesta, Valéria Buccheri, Filip Horvat, Irena Jenickova, Michaela Procházková, Jan Procházka, Matyáš Pinkas, Jiří Nováček, , Petr Svoboda

This study identifies the DExD/H helicase domain of Dicer as critical for accurate miRNA biogenesis, showing its role in substrate recognition, cleavage, and strand selection in mammals.

bioRxiv · Apr 13, 2022 · PreprintMolecular Biology

MLL4 is required for the first embryonic collective cell migration whereas MLL3 is not required until birth

Deepthi Ashokkumar, Qinyu Zhang, Christian Much, Anita S. Bledau, Jun Fu, Konstantinos Anastassiadis, Adrian Francis Stewart, Andrea Kranz

The paper investigates the distinct developmental roles of MLL3 and MLL4 in mice, finding MLL4 is critical for early embryonic migration while MLL3 is needed for lung maturation.

bioRxiv · Dec 9, 2019 · PreprintDevelopmental Biology

The Tug1 Locus is Essential for Male Fertility

Jordan P. Lewandowski, Gabrijela Dumbović, Audrey R. Watson, Taeyoung Hwang, Emily Jacobs-Palmer, Nydia P. Chang, Christian Much, Kyle M. Turner, Christopher Kirby, Jana Felicitas Schulz, Clara-Louisa Müller, Nimrod Daniel Rubinstein, Abigail F. Groff, Steve C. Liapis, Chiara Gerhardinger, , John L. Rinn

The paper identifies Tug1 as essential for male fertility, showing it has three distinct regulatory activities: a cis DNA repressor, a trans-acting lncRNA, and a conserved peptide affecting mitochondrial function.

bioRxiv · Feb 28, 2019 · PreprintGenomics

Terminal uridylyltransferases target RNA viruses as part of the innate immune system in animals

Jérémie Le Pen, Hongbing Jiang, Tomás Di Domenico, Emma Kneuss, Joanna Kosałka, Marcos Morgan, Christian Much, Konrad L. M. Rudolph, Anton J. Enright, Dónal O’Carroll, David Wang, Eric A. Miska

The paper identifies terminal uridylyltransferases as a conserved antiviral mechanism in animals by uridylating viral RNA to promote degradation.

bioRxiv · Dec 19, 2017 · PreprintImmunology