This study uses a genetic cross of Plasmodium falciparum isolates to analyze the roles of pfcrt and pm2/3 in piperaquine resistance and fitness traits.

bioRxiv · Jun 6, 2023 · PreprintGenomics

Optimizing bulk segregant analysis of drug resistance using Plasmodium falciparum genetic crosses conducted in humanized mice

Katelyn M. Vendrely, Xue Li, Sudhir Kumar, Elizabeth Delgado, Lisa A. Checkley, Douglas A. Shoue, Ann N. Reyes, Biley A. Abatiyow, Meseret T. Haile, Rupam Tripura, Thomas Julian Peto, Dysoley Lek, Katrina A. Button-Simons, Stefan H. I. Kappe, Mehul Dhorda, , Tim Anderson

This study optimizes bulk segregant analysis for identifying drug resistance loci in Plasmodium falciparum using genetic crosses in humanized mice, confirming the kelch13 locus and demonstrating the utility of cryopreserved pools.

iScience · Mar 16, 2022Peer-reviewedGenomics

Resolving within-host malaria parasite diversity using single-cell sequencing

Standwell C. Nkhoma, Simon G. Trevino, Karla M. Gorena, Shalini Nair, Stanley Khoswe, Catherine Jett, Roy Garcia, Benjamin J. Daniel, Aliou Dia, Dianne Janette Terlouw, Stephen Andrew Ward, Timothy J. C. Anderson, Ian H. Cheeseman

This study uses single-cell sequencing to analyze within-host diversity of Plasmodium falciparum in malaria patients, revealing up to seventeen unique lineages per patient and suggesting co-transmission from a single mosquito is more common than superinfection.

bioRxiv · Aug 13, 2018 · PreprintGenetics

High-resolution single-cell sequencing of malaria parasites

Simon G. Trevino, Standwell C. Nkhoma, Shalini Nair, Benjamin J. Daniel, Karla Moncada, Stanley Khoswe, Rachel L. Banda, François Henry Nosten, Ian H. Cheeseman

This paper develops a single-cell genomics protocol to reconstruct haplotypes of Plasmodium falciparum parasites and analyzes 48 cells from a malaria infection to estimate clonality and haplotype relatedness.

Genome Biology and Evolution · Dec 1, 2017Peer-reviewedGenomics