Thomas E. Hughes

Far-red fluorescent genetically encoded calcium ion indicators

This paper introduces two new far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, characterized for their Ca2+ sensitivity and performance in neuronal imaging.

Nirmatrelvir-Resistant Mutations in SARS-CoV-2 Mpro Enhance Host Immune Evasion via Cleavage of NF-κB Essential Modulator

The paper shows that Nirmatrelvir-resistant mutations in SARS-CoV-2 Mpro enhance immune evasion by cleaving NEMO, leading to reduced immune response and improved viral replication.

High throughput instrument to screen fluorescent proteins under two-photon excitation

The paper introduces a high-throughput instrument called GIZMO to screen fluorescent protein mutants under two-photon excitation, enabling efficient evolution of proteins optimized for this excitation method.

Live-cell assays for cell stress responses reveal new patterns of cell signaling caused by mutations in rhodopsin, α-synuclein and TDP-43

The paper uses live-cell assays to study how mutations in rhodopsin, α-synuclein, and TDP-43 affect cell stress and signaling, revealing altered Ca2+ signaling and phosphodiesterase activity.