Adam E. Cohen

Large-scale all-optical electrophysiology links excitability and connectivity to circuit dynamics in vivo

The paper develops tools for large-scale all-optical electrophysiology to study neural circuit dynamics, excitability, and connectivity in vivo.

Electrophysiology in nanoscale compartments

This paper investigates how voltage dynamics and ion content in nanoscale compartments differ from larger structures by adapting conductance-based models and simulating small vesicles and macrophage endosomes.

Mapping absolute membrane voltage using dynamic photocycle control

This paper introduces VMAP, a method for absolute membrane voltage imaging, and demonstrates its application in measuring drug effects, bioelectric patterns, and 3D voltage maps in zebrafish embryos.

Ether lipids influence cancer cell fate by modulating iron uptake

This study shows that ether lipids in cancer cells regulate membrane properties to influence iron uptake, ferroptosis, and metastasis, offering a therapeutic target.

A dendrite-resolved, in vivo transfer function from spike patterns to dendritic Ca2+

This study measures the voltage-to-Ca2+ transfer function in hippocampal CA2 pyramidal neurons during behavior and develops a biophysics-inspired model to predict Ca2+ signals from voltage waveforms.

Dendritic excitations primarily mediate back-propagation in CA1 pyramidal neurons during behavior

This study maps dendritic membrane potential dynamics in CA1 neurons during behavior, showing dendritic spikes are driven by back-propagating action potentials and influence spiking outputs and plasticity.

DENDRO: Recovery and denoising of whole-tree dendritic voltagefrom 2D voltage movies

DENDRO recovers and denoises 3D dendritic voltage from 2D voltage movies using a microscope model and a self-supervised neural network, achieving high accuracy on simulated and real data.

Optical segmentation-based compressed readout of neuronal voltage dynamics

This paper introduces a novel optical segmentation method for compressed readout of neuronal voltage dynamics, achieving high-speed recording of subcellular activity with improved data efficiency.

Optical single-channel recording via diffusional confinement in membrane tethers

The paper demonstrates a method to enhance single-channel transport detection by confining substrate diffusion in membrane tethers, enabling observation of individual Ca2+ transport events.

Far-red fluorescent genetically encoded calcium ion indicators

This paper introduces two new far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, characterized for their Ca2+ sensitivity and performance in neuronal imaging.

Optogenetic control of Nodal signaling patterns

This study develops optogenetic tools to control Nodal signaling patterns in zebrafish embryos, enabling precise spatial manipulation of signaling activity and rescue of developmental defects.

Mechanism of giant magnetic field effect in fluorescence of mScarlet3, a red fluorescent protein

The paper investigates the giant magnetic field effect in mScarlet3 fluorescence in E. coli, identifying FMN and light as factors restoring the effect and proposing a model for its mechanism.

Luminos: open-source software for bidirectional microscopy

Luminos is an open-source MATLAB library for bidirectional microscopy that enables precise synchronization of stimulation, imaging, and experimental parameters.

Swimming motions evoke Ca2+ events in vascular endothelial cells of larval zebrafish via mechanical activation of Piezo1

This study shows that swimming motions in larval zebrafish trigger Ca2+ events in vascular endothelial cells through mechanical activation of Piezo1, independent of neural activity or blood flow.

Dendritic excitations govern back-propagation via a spike-rate accelerometer

The paper investigates dendritic excitations in CA1 pyramidal neurons, showing how locally generated Na+ spikes drive history-dependent spike back-propagation and influence associative plasticity.

Tools for intersectional optical and chemical tagging on cell surfaces

The paper introduces photo-click adapters for optical and chemical cell surface tagging, demonstrating applications in zebrafish embryo imaging and combinatorial cell labeling.

Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators

The paper investigates the photophysics of FRET-opsin voltage indicators and demonstrates two-photon voltage imaging in live mice using optimized protocols.

All-optical mapping of Ca2+ transport and homeostasis in dendrites

The paper develops an all-optical system to measure calcium transport properties in dendrites, finding a mean length constant of ~5.8 m and a half-life of ~1.7 s.

Optical constraints on two-photon voltage imaging

The paper compares one-photon and two-photon voltage imaging, finding that 2P requires much higher illumination power and faces tradeoffs between noise and tissue damage.

Solaris: a panel of bright and sensitive hybrid voltage indicators for imaging membrane potential in cultured neurons

The paper introduces Solaris, a hybrid voltage indicator with improved sensitivity for imaging membrane potential in cultured neurons.

Mapping uterine calcium dynamics during the ovulatory cycle in live mice

The paper maps uterine calcium dynamics in live mice across the estrous cycle using a calcium reporter and imaging, revealing phase-dependent initiation patterns of uterine excitations.

A phenotypic screening platform for chronic pain therapeutics using all-optical electrophysiology

The paper presents an in vitro model of OA pain using SPARC and a high-throughput all-optical electrophysiology system to screen drugs for pain relief.

Mapping memories: pulse-chase labeling reveals AMPA receptor dynamics during memory formation

The paper introduces EPSILON, a technique to map AMPA receptor dynamics during memory formation and finds a correlation between synaptic plasticity and cFos expression.

Voltage dynamics of dendritic integration and back-propagation in vivo

The paper measures voltage dynamics in dendrites of mice neurons during synaptic input integration and action potential propagation, finding shared membrane voltage and rate-dependent propagation of back-propagating action potentials.

Time-tagged ticker tapes for intracellular recordings

The paper develops a protein-based ticker tape system to record neural activation histories with high temporal resolution.

Ketamine modulates a norepinephrine-astroglial circuit to persistently suppress futility-induced passivity

The paper shows ketamine suppresses futility-induced passivity in zebrafish by modulating astroglial calcium through norepinephrine signaling.

A bioelectrical phase transition patterns the first beats of a vertebrate heart

The paper identifies a bioelectrical phase transition as the mechanism initiating the first heartbeat in zebrafish, using optogenetic perturbations and all-optical electrophysiology.

Statistically unbiased prediction enables accurate denoising of voltage imaging data

SUPPORT is a self-supervised method that denoises voltage imaging data by leveraging spatiotemporal dependencies, preserving dynamics without requiring action potential information.

Membrane tension propagation couples axon growth and collateral branching

This paper shows that membrane tension propagates along axons at 20 micrometers per second, influencing axon growth and branching through mechanical signaling.

Dendritic branch structure compartmentalizes voltage-dependent calcium influx in cortical layer 2/3 pyramidal cells

The paper shows that specific dendritic branches in cortical layer 2/3 pyramidal cells fail to evoke calcium influx from bAPs due to branch-specific properties, despite having functional VGCCs.