Pavel Vaňáček

CascadeMAP: Autonomous Closed-loop Optimization of Enzyme Cascades via Microfluidics, Machine Learning and Agentic AI

Unattended droplet-microfluidic Bayesian optimization of a glycerol detection cascade matched design of experiments (DoE) with one third of the conditions; a second cascade used DoE with Raman readout.

Uncovering Functional Distant Mutations by Ultra-High-Throughput Screening of Dehalogenases

This study uses ultrahigh-throughput screening to identify mutations in dehalogenases that modulate substrate access and binding, revealing distal functional effects on enzyme dynamics.

Taurine Inhibits Apolipoprotein E4 Aggregation

This study shows taurine inhibits ApoE4 aggregation and reduces its pathophysiological effects, similar to tramiprosate and 3-sulfopropanoic acid.

Investigating the Conformational Flexibility of Staphylokinase Across Multiple Time Scales

This study investigates the conformational flexibility of staphylokinase variants to explain their reduced immunogenicity and altered properties.