The paper develops a genetically encoded voltage indicator to measure subthreshold voltage dynamics in neurons, revealing electrical diversity and local computation.

bioRxiv · May 31, 2026 · PreprintNeuroscience

Photon-Resolved Excitation-Denoised (PRED) Three-Photon Imaging Improves Detection of Neuronal Activity in Awake and Behaving Mice

Tiberiu S. Mihaila, Eunji Kong, Adrian Negrean, Tristan C. Geiller, Darcy S. Peterka, Attila Losonczy

The paper improves three-photon imaging for neuronal activity detection in behaving mice by reducing noise and increasing imaging speed.

bioRxiv · May 24, 2026 · PreprintNeuroscience

Glutamate indicators with increased sensitivity and tailored deactivation rates

Abhi Aggarwal, Adrian Negrean, Yang Chen, Rishyashring R. Iyer, Daniel Reep, Anyi Liu, Anirudh Palutla, Michael E. Xie, Bryan J. MacLennan, Kenta M. Hagihara, Lucas W. Kinsey, Julianna L. Sun, Pantong Yao, Jihong Zheng, Arthur Tsang, , Kaspar Podgorski

The paper develops two variants of a glutamate indicator with enhanced sensitivity and tailored deactivation rates for studying synaptic transmission in vivo.

Nature Methods · Dec 23, 2025Peer-reviewedNeuroscience

Functional architecture of intracellular oscillations in hippocampal dendrites

Zhenrui Liao, Kevin C. Gonzalez, Deborah M Li, Catalina M. Yang, D. J. Holder, Natalie E. McClain, Guofeng Zhang, Stephen W. Evans, Mariya Chavarha, Jane Simko, Christopher D. Makinson, Michael Z. Lin, Attila Losonczy, Adrian Negrean

The paper uses two-photon imaging and electrophysiological recordings to study dendritic membrane potential dynamics in mouse hippocampal pyramidal cells during behavior, revealing spatial and temporal patterns of electrical signaling.

Nature Communications · Jul 26, 2024Peer-reviewedNeuroscience

Synaptic Basis of Behavioral Timescale Plasticity

Kevin C. Gonzalez, Adrian Negrean, Zhenrui Liao, Franck Polleux, Attila Losonczy

The paper experimentally tests a model of synaptic plasticity in awake mice, showing asymmetric potentiation and depression of synaptic inputs around plateau spikes and compartment-specific differences in plasticity.

bioRxiv · Oct 4, 2023 · PreprintNeuroscience

A positively tuned voltage indicator reveals electrical correlates of calcium activity in the brain

Stephen W. Evans, Dongqing Shi, Mariya Chavarha, Mark H. Plitt, Jiannis Taxidis, Blake A. Madruga, Jiang Lan Fan, Fuu-Jiun Hwang, Siri Camee van Keulen, Carl‐Mikael Suomivuori, Michelle M. Pang, Sharon J. Su, Sungmoo Lee, Yukun Alex Hao, Guofeng Zhang, , Michael Z. Lin

This paper introduces positively tuned GEVIs that enhance spike detection in vivo with standard imaging systems, offering higher temporal resolution than calcium indicators.

Nature Methods · Jul 1, 2023Peer-reviewedNeuroscience

Proximity of astrocyte leaflets to the synapse determines memory strength

Aina Badia‐Soteras, Tim S. Heistek, Mandy S. J. Kater, Aline Mak, Adrian Negrean, Michel C. van den Oever, Huibert D. Mansvelder, Baljit S. Khakh, Rogier Min, August Benjamin Smit, Mark H. G. Verheijen

This study shows that astrocyte leaflet structure, regulated by Ezrin, affects memory strength by controlling glutamate diffusion and synaptic function in the hippocampus.

Biological Psychiatry · Oct 29, 2022Peer-reviewedNeuroscience

Somatic Depolarization Enhances Hippocampal CA1 Dendritic Spike Propagation and Distal Input Driven Synaptic Plasticity

Tobias Bock, Adrian Negrean, Steven A. Siegelbaum

Journal of Neuroscience · Mar 7, 2022Peer-reviewedNeuroscience

Compartment-specific tuning of hippocampal dendritic feature selectivity by intracellular Ca2+ release

Justin K. O’Hare, Yusuke Hirabayashi, Victoria L. Hewitt, Heike Blockus, Miklos Szoboszlay, Sebi V. Rolotti, Tristan C. Geiller, Adrian Negrean, Vikas Chelur, Attila Losonczy, Franck Polleux

The paper investigates the role of intracellular Ca2+ release in shaping dendritic feature selectivity in hippocampal neurons during spatial navigation.

bioRxiv · Sep 27, 2021 · PreprintNeuroscience