Evžen Bouřa

A Kiss-and-Ride Mechanism for Lipid Exchange at Membrane Contact Sites

This study identifies a 'kiss-and-ride' mechanism for lipid transfer mediated by Osh6, showing that transient membrane tethering facilitates cargo extraction and transport.

Characterization of the N7 RNA cap methyltransferase from Trichomonas vaginalis and inhibitor discovery

The paper functionally and structurally characterizes RNA cap methyltransferases from Trichomonas vaginalis and identifies inhibitors targeting these enzymes.

Branched C7-Substituted 7-Deaza-SAH Analogues Occupy the Entire SAM-Binding Pocket of Mpox Virus VP39 and Dengue Virus NS5 Methyltransferases

The paper develops branched C7-substituted 7-deaza-SAH analogues that inhibit mpox virus VP39 and dengue virus NS5 methyltransferases by occupying their SAM-binding pockets.

Structure of the Hibernating Francisella tularensis Ribosome and Mechanistic Insights into Its Inhibition by Antibiotics

This study determines the structure of the hibernating F. tularensis ribosome and shows RaiA and antibiotics bound to it, offering insights for therapeutic intervention.

Catalytic and Structural Insights into Neil3-Dependent Unhooking of Endogenous Abasic DNA Crosslink

This study clarifies the mechanism of NEIL3 in unhooking Ap-ICLs by showing that a processed form of NEIL3 efficiently removes crosslinks without generating strand breaks.

Mechanistic Insights into Alcohol-Induced DNA Crosslink Repair by the Fanconi Anemia Nuclease Slx4-Xpf-Ercc1

This study identifies the role of the SLX4-XPF-ERCC1 nuclease complex in repairing alcohol-induced DNA crosslinks by making two precise incisions around the lesion.

A recombinant expression system for the Plasmodium falciparum proteasome enables structural analysis of its assembly and the design of selective inhibitors

This paper develops a recombinant Pf20S platform for structural analysis and inhibitor design, identifying a selective inhibitor with high potency against Plasmodium falciparum proteasome.

Structural insights into Salinosporamide A mediated inhibition of the human 20S proteasome

The paper presents the cryo-EM structure of the human 20S proteasome bound to marizomib, revealing its binding mode and irreversible inhibitory mechanism.

Structural and functional insights in flavivirus NS5 proteins gained by the structure of Ntaya virus polymerase and methyltransferase

The paper structures and biochemically analyzes the MTase and RdRp domains of Ntaya virus NS5, showing conserved enzymatic centers across Flaviviridae but differing MTase activities.

Fluorinated cGAMP analogs, which act as STING agonists and are not cleavable by poxins: structural basis of their function

This study designs and synthesizes fluorinated cGAMP analogs that are stable against poxin cleavage and show high STING activation, with structural insights into their binding modes.

Substrate specificity of SARS-CoV-2 nsp10-nsp16 methyltransferase

The paper identifies five capped RNA substrates for SARS-CoV-2 nsp10-nsp16 methyltransferase using an LC-MS-based method and determines the IC50 of sinefungin.

Structural analysis of the SARS-CoV-2 methyltransferase complex involved in coronaviral RNA cap creation

The paper solves the crystal structure of the SARS-CoV-2 nsp10-nsp16 MTase complex and models its interaction with RNA, revealing low conservation with Zika MTase and implications for inhibitor design.