Bettina Siebers

A TFIIB paralog drives cyclic transcription to orchestrate the archaeal cell cycle

This study identifies TFB2, a TFIIB paralog, as a key regulator of cyclic transcription in Sulfolobus acidocaldarius, linking it to cell cycle progression without CDKs.

Deletion of the gene for a cyanobacterial ribosome-associated protein affects the carbon/nitrogen metabolism

This paper characterizes the function of the cyanobacterial gene ssr3189 by analyzing its deletion mutant, revealing its role in linking translation with metabolic control and RNA decay.

Story about honest mistakes: The cyanobacterium Synechocystis has a promiscuous Entner-Doudoroff (ED) aldolase but no functional ED pathway.

This study revisits the claim of the Entner-Doudoroff pathway in Synechocystis, showing that while the aldolase is present, the full pathway is absent due to missing enzymes and experimental errors.

Establishment of an efficient one-step enzymatic synthesis of cyclic-2,3-diphosphoglycerate

This paper develops an efficient one-step enzymatic method to synthesize cyclic-2,3-diphosphoglycerate using MfcDPGS from Methanothermus fervidus, achieving high conversion efficiency and scalability.

Glycerol degradation in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius involves an unusual glycerol-3-phosphate dehydrogenase

This study identifies an unusual glycerol-3-phosphate dehydrogenase in Sulfolobus acidocaldarius involved in glycerol degradation, showing its essential role in growth on glycerol.

Fructose-1,6-bisphosphatase (FBPase) fine-tunes heterotrophic growth in cyanobacteria

This study identifies FBPase in Synechocystis as a monofunctional enzyme that supports heterotrophic growth without redox regulation, distinct from the bifunctional F/SBPase.

5'-untranslated region sequences enhance plasmid-based protein production in Sulfolobus acidocaldarius

The paper shows that adding 5'-UTRs from S. acidocaldarius improves plasmid-based protein production, with the alba 5-UTR increasing yield fourfold.

The archaeal family 3 polyphosphate kinase reveals a function of polyphosphate as energy buffer under low energy charge

This study identifies and characterizes a novel polyphosphate kinase in Sulfolobus acidocaldarius, revealing polyphosphate's role as an energy buffer under low energy charge.

De novo synthesis of fatty acids in Archaea via an archaeal fatty acid synthase complex

This study shows that archaea can synthesize fatty acids using a newly identified pathway and enzyme complex, providing insights into their evolutionary lipid composition.