Stefanie Kaiser

The paper benchmarks cross-platform RNA modification quantification using LC-MS/MS and finds that harmonized workflows and SILIS normalization improve reproducibility across different instrument types.

The paper benchmarks MS-Seq for human 28S rRNA analysis, showing reproducible results across platforms but highlighting gaps in coverage and ambiguity.

This study evaluates the long-term stability of RNA nucleoside standards under different storage conditions and proposes guidelines for their preparation and quality control.

The paper shows that host tRNA modifications are critical for efficient influenza A virus translation and antiviral responses, with ELP3 loss impairing viral propagation.

This study assesses ALKBH1 activity in vitro and in human cell lines using mass spectrometry, finding no significant impact on tRNA modifications under optimal conditions.

This study maps epitranscriptomic changes in E. coli under oxidative and acid stress, identifying RNA modifications that influence stress adaptation and survival.

The paper introduces a sensitive oligonucleotide-MS workflow for analyzing native RNA modifications and benchmarks three nucleases for their ability to cleave modified RNAs.

This study uses NAIL-MS to investigate ALKBH5's role in m6A demethylation in vivo, finding no significant effect on m6A dynamics or spatial distribution in HEK 293T cells.

The paper characterizes phosphorothioate (PT) modifications in the human gut microbiome using metagenomics and mass spectrometry, revealing temporal dynamics and species-specific patterns.

This study uses direct RNA sequencing to analyze RNA modifications in E. coli under heat stress, revealing changes in m5C, m6A, and m6,6A levels in rRNA and shifts in mRNA modification patterns.

The paper identifies the roles of TRMT1 and TRMT1L in tRNA modifications, showing their impact on tRNA stability and function, and links TRMT1 variants to disease.

This study shows that tRNA hypomodification in Aspergillus fumigatus leads to 5-fluorocytosine resistance through premature activation of a cross-pathway control system.

This study identifies genetic interactions among tRNA modification genes in E. coli K12, revealing synthetic lethal pairs and condition-dependent phenotypes.

This study investigates RNA modification changes in HEK293T cells under 5-fluorouracil treatment using NAIL-MS, revealing dynamic modifications in tRNA and rRNA.

The paper reports the cryo-EM structure of METTL6 in complex with SerRS and tRNASer, revealing their interaction and methylation mechanism.

This study identifies NAD-capped RNAs during T4 phage infection of E. coli, showing dynamic changes in NAD-capping and revealing a phage-encoded Nudix hydrolase involved in NAD-RNA decapping.

This study compares the effects of azacytidine and decitabine on an acute myeloid leukemia cell line, finding that decitabine induces apoptosis while azacytidine does not, linked to differences in hydroxy-methyl-dC levels.