Samir EL Andaloussi

This study shows that chemical modifications to siRNA and linkers in AOCs can improve potency and tissue targeting while allowing higher payload loading.

This study compares lipid-conjugated ASO delivery in mice, showing differential tissue and CNS targeting based on lipid type and administration route.

This study develops bioengineered extracellular vesicles that neutralize pneumolysin and reduce inflammation in pneumococcal meningitis, improving survival in a mouse model.

This study identifies TSPAN3 as a marker of a distinct secretory multivesicular body population with unique trafficking and EV composition compared to CD63-positive compartments.

This paper demonstrates efficient genome editing in non-human primate brains using engineered extracellular vesicles, achieving therapeutic potential for neurological disorders.

This study shows that exon skipping therapy is more effective when started early in adult mdx mice than in aged mice, leading to better dystrophin restoration and transcriptomic changes.

This study develops an engineered extracellular vesicle platform that improves shRNA loading and delivery efficiency, achieving robust gene silencing in multiple cell types and the mouse brain.

This study develops a click chemistry-based strategy for siRNA conjugation to enhance extrahepatic RNAi, finding that bis-fatty-acid conjugates improve activity in heart and muscle while showing modality-specific limitations.

The paper introduces Nano-SMF, a nanofluidic method for size and 3-color fluorescence phenotyping of single nanoparticles, demonstrating improved accuracy over existing techniques for EV analysis.

This study shows that systemic inflammation significantly prolongs EV plasma half-life and enhances their accumulation in inflamed organs like the liver and spleen.

This study investigates the role of tetraspanins in EV bioengineering by knocking out CD9, CD63, and CD81 in HEK293T cells and shows increased engineered EV production upon reintroduction of tetraspanin-fusion constructs.

This paper develops intein-engineered virus-like particles for efficient gene editing delivery, achieving robust gene editing in multiple cell types and organs.

This paper develops hPep peptide-based nanoparticles for systemic mRNA delivery, showing effective tissue distribution and protein expression in mice and non-human primates.

This paper develops a modular strategy for delivering CRISPR-Cas9 via extracellular vesicles using aptamer-based loading and UV-activated cargo release, demonstrating efficient delivery and versatility in Cas9 variants.

The paper combines experimental and computational methods to model how uORFs regulate translation, identifying key features and interactions that influence their repressive activity.

This paper develops hPep-based nanoparticles for efficient delivery of gene editors across multiple cell types, achieving high editing efficiency in human cells.

This study demonstrates elasto-inertial focusing of sub-25 nm particles in straight microchannels, enabling high-resolution separation of biological nanoparticles for biomedical applications.

This study develops a method to accurately measure the elastic modulus of extracellular vesicles, showing that surface adhesion and membrane fluctuations significantly affect the results.

This study investigates N-acetylcysteine amide (NACA) as a prophylactic treatment for acute GvHD in a mouse model, showing reduced disease severity and improved survival without affecting donor engraftment.

This study identifies histones as exosome membrane proteins regulated by cell stress, showing they are secreted via the exosome pathway and lack nuclear post-translational modifications.

The paper introduces iSPP, an imaging-based method for analyzing nanoscale bioparticles using conventional confocal microscopy and provides software for its application.

This paper introduces CRISPR MiRAGE, a miRNA-sensing guide RNA system that enables tissue-specific gene editing by modulating CRISPR activity based on miRNA signatures.

This study shows that SARS-CoV-2 and HSV-1 induce amyloid aggregation of proteins in human CSF, leading to soluble protein depletion, suggesting a potential mechanism linking viral infections to neurodegenerative diseases.

This paper introduces a method to map EV surfaceomes and target cells using APEX2 proximity labeling on EVs, revealing novel EV-sorting domains.

This paper develops a PTTG1IP-based platform for efficient extracellular vesicle (EV) cargo delivery, showing improved therapeutic delivery compared to CD63-based scaffolds.

This paper introduces a Snorkel-tag system for affinity purification of extracellular vesicles using CD81 fused with tags and transmembrane domains, enabling non-destructive isolation and future applications in tracing and analyzing EVs.

The paper identifies 24 novel scaffold proteins with conserved EV-sorting abilities, outperforming CD63 in cargo loading and demonstrating improved EV engineering potential.

This study shows GAPDH facilitates EV biogenesis and enables efficient siRNA delivery to the brain, reducing huntingtin gene expression in a mouse model.

The paper shows that most extracellular miRNAs are secreted non-vesicularly, with only <2% associated with EVs, even though EVs can stabilize miRNAs in serum.

This study engineers extracellular vesicles with IL6ST decoy receptors to inhibit the IL6 trans-signalling pathway, showing efficacy in vitro and in a Duchenne muscular dystrophy mouse model.