Patrick A. Limbach

The paper benchmarks cross-platform RNA modification quantification using LC-MS/MS and finds that harmonized workflows and SILIS normalization improve reproducibility across different instrument types.

The paper benchmarks MS-Seq for human 28S rRNA analysis, showing reproducible results across platforms but highlighting gaps in coverage and ambiguity.

This study examines how low-protein and high-fat diets affect tRNA abundance and modifications in multiple mouse tissues using RNA mass spectrometry and OTTR-seq.

This study shows that microbiome-derived queuosine (Q) affects tyrosine metabolism and predator avoidance in mosquito larvae, leading to impaired behavior and survival.

This study reveals the structural mechanism of human lysyl-tRNA synthetase aminoacylation of modified tRNALys3 using cryo-EM structures.

This study identifies TRMT1L as the methyltransferase responsible for m22G27 modification on tRNA-Tyr-GUA, showing its role in translation efficiency and oxidative stress resistance.

The paper examines tRNA abundance and modifications in Aedes aegypti during blood-feeding and vitellogenesis, finding increased tyrosine tRNA transcription is critical for egg yolk protein synthesis.

This study identifies m6A RNA methylation by Mettl3 as essential for developmental pausing in mice by maintaining transcriptional dormancy through mRNA destabilization and suppression of N-Myc.

This paper develops a sensitive nLC-MS method using capillary porous graphitic carbon columns to quantify chemically modified ribonucleosides at attomol levels in complex samples.