Upasna Sharma

Quantitative CDK2 Dynamics Are Linked to Cell Fate Decisions in Differentiating Trophoblast Stem Cells

The paper shows CDK2 activity levels correlate with different cell fate outcomes in differentiating trophoblast stem cells.

A sperm-enriched 5'fragment of tRNA-Valine regulates preimplantation embryonic transcriptome and development

This study identifies a sperm-enriched tRFValCAC that regulates early embryonic mRNA splicing and preimplantation development through interactions with hnRNPAB.

Tracking tRNA evolution in closely related mouse strains

The paper analyzes tRNA gene variation across 17 closely related mouse strains to understand evolutionary dynamics and identifies conserved and strain-specific tRNA features.

A survey of dietary effects on tRNA abundance and modifications

This study examines how low-protein and high-fat diets affect tRNA abundance and modifications in multiple mouse tissues using RNA mass spectrometry and OTTR-seq.

Epididymis-specific RNase A family genes regulate fertility and small RNA processing

This study shows that epididymis-specific RNase A family genes are essential for fertility and regulate small RNA processing in sperm.

An atlas of cell types in the mammalian epididymis and vas deferens

The paper creates a single-cell atlas of the murine epididymis and vas deferens, identifying cell types and their regional specialization, and revealing potential signaling networks.

Small RNAs are trafficked from the epididymis to developing mammalian sperm

The paper shows that small RNAs in mature mammalian sperm originate from epididymal somatic cells, not testicular germ cells, and are delivered via vesicular transport during post-testicular maturation.

A synthetic biology approach to probing nucleosome symmetry

The paper introduces a synthetic biology approach to study nucleosome symmetry by creating asymmetric H3 heterodimers and demonstrates their utility in analyzing histone modification crosstalk.