Adam M. Deutschbauer

The paper uses phages to manipulate Variovorax species in microbiomes, confirming thiamine interdependencies and laying groundwork for targeted microbiome research.

The paper identifies and validates novel plasmid origins of replication from an environmental Pantoea strain, enabling expression of heterologous proteins and pathways in both model and non-model bacteria.

The paper characterizes bacterial cobamide preferences by testing growth under specific cobamide-dependent conditions, revealing broad diversity in cobamide usage across organisms and metabolic pathways.

The paper creates a mutant fitness compendium for Bifidobacterium breve to identify molecular factors affecting colonization and host-microbe interactions.

The paper identifies a novel phenylpropanoid methyl esterase that catabolizes aromatic biological nitrification inhibitors, enabling their use as carbon sources by bacteria.

The paper updates GapMind to include novel enzymes for amino acid biosynthesis, reducing pathway gaps in prokaryotes from 1.4 to 0.7 per genome.

The paper introduces Coaux-Seq, a method using DNA barcoded fragment libraries and auxotroph complementation to characterize protein function in bacteria, identifying 53 previously unvalidated proteins.

This paper uses high-throughput genetics to identify nutrient utilization and accessory energy metabolism genes in the methanogen Methanococcus maripaludis.

The paper identifies a tailocin, a phage-derived element, used by Pseudomonas spp. to suppress competitors in metapopulations, showing its persistence over 170 years.

This study investigates how genomic and environmental factors influence the distribution of Castellaniella species in a contaminated subsurface, identifying genetic mechanisms underlying their adaptation to stress.

The paper identifies a small RNA, MasB, that increases Bacteroides thetaiotaomicron tolerance to tetracyclines by modulating gene expression under antibiotic stress.

This study evaluates how different inoculation practices and harvesting times affect microbial community reproducibility in a fabricated ecosystem with Brachypodium distachyon.

This study identifies two genes in a two-component system whose deletion increases PHB productivity in Cupriavidus basilensis under non-stress conditions.

This study measures ion thresholds for phage infection and shows that phage impact on terrestrial microbiomes is strongest in high-ion environments with simple carbon sources.

The paper identifies bacterial strains capable of degrading sorgoleone, a plant exudate, and characterizes the genetic basis for this trait, offering potential for engineering beneficial plant-microbe interactions.

This paper develops a fast, cheap protocol for assembling ordered transposon insertion libraries in anaerobic bacteria, using cell sorting and barcoded transposons to improve accuracy and efficiency.

The paper identifies gene deletions that enhance indigoidine production in Pseudomonas putida using fitness profiling and bioreactor experiments.

GapMind annotates amino acid biosynthesis in bacteria and archaea by using experimentally characterized proteins and genetic data from minimal media-growing microbes.

The paper validates a native plasmid-encoded mercury resistance operon in groundwater bacteria and identifies strains capable of natural transformation.

This study identifies and disrupts genes in Pseudomonas putida that cause unwanted metabolism of valerolactam, increasing its production titer.

The paper introduces a method called 'magic pools' to efficiently assess transposon delivery vectors in bacteria by using barcoded libraries and sequencing to identify effective vectors for mutagenesis.