Andrea Graziadei

Accelerating discovery and integration from RNA to Protein with R2T2P

R2T2P integrates transcriptomics and proteomics data to study FSHD, revealing novel RNAs and their impact on translation and protein levels.

Evolutionary diversification of lipid logistics shapes synaptic maturation in primates

This study compares human and chimpanzee neurons to show that human neurons have altered lipid metabolism and trafficking, contributing to delayed synaptic maturation during brain development.

Molecular basis of tRNA modification by the human m5C methyltransferase NSUN2

This study determines the structure of the NSUN2-tRNA complex and reveals how NSUN2 recognizes and modifies tRNA through RNA architecture and specific amino acid interactions.

Condensin II activation by M18BP1

The paper identifies M18BP1 as a factor required for condensin II localization to chromatin and shows it competes with MCPH1 to activate condensin II during mitosis.

An NADH-controlled gatekeeper of ATP synthase

The paper identifies AIFM1/AK2 interaction as a gatekeeper of mitochondrial ATP synthase, showing it is NADH-dependent and linked to metabolic state, with implications for mitochondrial diseases.

Structural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters

The paper presents cryo-EM structures of the SNAPc-containing Pol III PIC on the U6 snRNA promoter and identifies structural differences in SNAPc engagement between Pol III and Pol II PICs.

Molecular basis of mRNA delivery to the bacterial ribosome

This study investigates how bS1 facilitates mRNA delivery to the ribosome and enhances translation initiation by RNAP in bacteria.

Protein Complexes in cells by AI-assisted structural proteomics

The paper uses AI to predict protein complex structures in Bacillus subtilis using crosslinking and cofractionation mass spectrometry data.

SARS-CoV-2 Nsp1 N-terminal and linker regions as a platform for host translational shutoff

This study identifies the role of Nsp1's N-terminal and linker regions in host translational shutoff by mapping their interactions with ribosomal and eIF3 components.

In-cell architecture of an actively transcribing-translating expressome

The paper determines the in-cell architecture of a transcribing and translating expressome in Mycoplasma pneumoniae using integrative structural methods.