bioRxivBiochemistry
DeGlyPHER: an ultrasensitive method for analysis of viral spike N-glycoforms
S. Baboo, J. K. Diedrich, S. Martinez-Bartolome, X. Wang, T. Schiffner, B. Groschel, W. R. Schief, J. C. Paulson, J. R. Yates
Abstract
Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover immune epitopes. We have developed an ultrasensitive "single pot" method to assess glycan occupancy and the extent of glycan processing from high-mannose to complex forms at each N-glycosylation site. Though aimed at characterizing glycosylation of viral spike-proteins as potential vaccines, this method is applicable for analysis of site-specific glycosylation of any glycoprotein.
- organism: virus
- assay: proteomics
- research focus: protein folding & dynamics
- approach: experimental assay
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