P. Azadi

Structure and mechanism of biosynthesis of Streptococcus mutans cell wall polysaccharide

This study identifies the structure and biosynthesis mechanism of Streptococcus mutans cell wall polysaccharide, revealing key modifications and their roles in bacterial morphology and biofilm formation.

Mutant GNAS drives a pyloric metaplasia with tumor suppressive glycans in intraductal papillary mucinous neoplasia

This study identifies GNASR201C as a driver of pyloric metaplasia in IPMN, characterized by tumor-suppressive glycans that inhibit cancer progression.

A novel exopolysaccharide pathway from a freshwater Sphingomonas isolate

The paper identifies a novel exopolysaccharide (EPS) pathway in Sphingomonas sp. LM7, showing it produces a unique linear polymer with 3- and 4-linked glucose, galactose, and glucuronic acid residues.

Plasma Glycomic Markers of Accelerated Biological Aging During Chronic HIV Infection

This study identifies glycomic alterations in HIV-positive individuals that correlate with accelerated aging and comorbidities, suggesting potential biomarkers for early detection.

Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxication

This study identifies fucosylated glycoproteins and glycolipids as opposing factors in cholera toxin binding and intoxication in intestinal cells.

Permethylation as a Strategy for High Molecular Weight Polysaccharide Structure Analysis by NMR& Case Study of Xylella fastidiosa EPS

The paper introduces a permethylation strategy for NMR analysis of high molecular weight polysaccharides, applied to Xylella fastidiosa EPS to overcome solubility and structural complexity challenges.

A weaponized phage suppresses competitors in historical and modern metapopulations of pathogenic bacteria

The paper identifies a tailocin, a phage-derived element, used by Pseudomonas spp. to suppress competitors in metapopulations, showing its persistence over 170 years.

Siglecs in the Porcine Oviduct and Sialylated Ligands on Sperm: Roles in the Formation of the Sperm Reservoir

This study identifies Siglecs and sialylated ligands on porcine sperm that may mediate immune tolerance in the oviduct, contributing to sperm reservoir formation.

Comparative Mucomic Analysis of Three Functionally Distinct Cornu aspersum Secretions

This study compares the composition and properties of three mucus types from Cornu aspersum to understand their structure-function relationships and identify novel components.

Post-translational modification by the Pgf glycosylation machinery modulates Streptococcus mutans physiology and virulence

This study shows that the Pgf glycosylation machinery in Streptococcus mutans modifies surface proteins, affecting biofilm formation and virulence, with phosphorylation compensating for glycosylation loss.

Surface anchoring of the Kingella kingae galactan is dependent on the lipopolysaccharide O-antigen

This study identifies the mechanism by which Kingella kingae galactan is anchored to the bacterial surface via an interaction with an atypical lipopolysaccharide O-antigen.

The Colon Mucosal Sialylglycome Is Redox-Regulated by the Golgi Enzyme QSOX1

This study shows that QSOX1 regulates sialylation of the colon glycome by forming disulfides in Golgi glycosyltransferases, not by catalyzing mucin crosslinking.

Metabolic heritage mapping: heterogenous pools of cytoplasmic nucleotide sugars are selectively utilized by various glycosyltransferases

The paper shows that cells use different pools of GDP-fucose for glycosylation, depending on the origin of the monosaccharide, and that this selection is tightly regulated.

SARS-CoV-2 Nucleocapsid protein is decorated with multiple N- and O-glycans.

This study identifies multiple N- and O-glycans on the SARS-CoV-2 nucleocapsid protein, providing structural insights for vaccine design and understanding viral pathogenesis.

Structure and function of a novel osmoregulated periplasmic fiber-forming high-molecular-weight carbohydrate of Myxococcus xanthus

This paper identifies a novel high-molecular-weight fiber-forming polysaccharide in Myxococcus xanthus that functions as an osmoregulated periplasmic glucan, replacing canonical OPGs and stabilizing the cell envelope during spore formation.

Structural basis of Blastomyces Endoglucanase-2adjuvancy in anti-fungal and -viral immunity

This paper identifies Bl-Eng2 as an adjuvant that enhances cell-mediated immunity against fungal and viral infections through structural and mechanistic insights.

Mass spectrometric method for the unambiguous profiling of cellular dynamic glycosylation.

This paper introduces a mass spectrometry-based method to directly detect non-natural sugars in glycopeptides, enabling the study of dynamic glycosylation without metabolic tagging.

Comprehensive characterization of N- and O- glycosylation of SARS-CoV-2 human receptor angiotensin converting enzyme 2.

This study characterizes N- and O-glycosylation of hACE2, identifying novel sites and glycan structures that may influence SARS-CoV-2 binding and entry.

Deducing the N- and O- glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2

This study identifies N- and O-glycosylation sites on SARS-CoV-2 spike protein subunits S1 and S2 using mass spectrometry and bioinformatics, revealing unexpected O-glycosylation on the RBD of S1.

Structure of the Xylan O-Acetyltransferase AtXOAT1 Reveals Molecular Insight into Polysaccharide Acetylation in Plants

The paper characterizes AtXOAT1, a plant xylan O-acetyltransferase, revealing its structural and mechanistic details for xylan acetylation.

Species-specific recognition of Sulfolobales mediated by UV inducible pili and S-layer glycosylation patterns

This study identifies species-specific regions in Sulfolobus pilin subunits and unique N-glycan patterns that mediate species-specific aggregation and DNA exchange.

Composition of the holdfast polysaccharide from Caulobacter crescentus

This study isolates and characterizes the holdfast polysaccharide from Caulobacter crescentus, identifying its monosaccharide composition and structural features.

Determining the link between alpha-gal-containing antigens in North American ticks and red meat allergy

This study identifies the presence of alpha-gal in the salivary glands of two tick species and links it to red meat allergy through IgE antibody responses.

CRISPR/Cas9 gene editing for the creation of an MGAT1 deficient CHO cell line to control HIV-1 vaccine glycosylation

This paper develops a CRISPR/Cas9-edited CHO cell line deficient in MGAT1 to produce HIV-1 gp120 with early glycosylation intermediates, enhancing binding to glycan-dependent broadly neutralizing antibodies.

Structure and function of accessory Sec proteins involved in the adhesin export pathway of Streptococcus gordonii

This study investigates the sequential O-glycosylation of an adhesin in Streptococcus gordonii and its interaction with accessory Sec proteins for export.

A library of structurally homogeneous human N-glycans synthesized from microbial oligosaccharide precursors

This paper describes a bioenzymatic method to synthesize homogeneous human N-glycans from microbial precursors, enabling efficient production of glycan structures for glycoscience applications.